Ali Damien, Sacchetto Emilie, Dumontet Erwan, Le Carrer Didier, Orsonneau Jean-Luc, Delaroche Odile, Bigot-Corbel Edith
Laboratoire de biochimie, Centre hospitalier universitaire de Nantes, Hôpital Guillaume et René Laënnec, Saint-Herblain, France.
Ann Biol Clin (Paris). 2014 May-Jun;72(3):297-311. doi: 10.1684/abc.2014.0952.
The study of the influence of hemolysis was determined experimentally for twenty two biochemical parameters on the analyzer Cobas 6000 ce (Roche Diagnostics). The addition method of hemolysate was used to create an increasing concentration of hemoglobin ranging from 0 to 2000 μmol/L. The limit of 10% variation was chosen to define the influence of hemolysis on the measurement. The parameters studied were classified into several categories: the parameters for which hemolysis does not influence the measurement: albumin, uric acid, calcium, C-reactive protein, myoglobin, NT -pro BNP, S100 protein, and urea; parameters impacted positively leading to an overestimation of the result: aspartate aminotransferase, total cholesterol, creatine kinase, creatinine, lactate dehydrogenase, magnesium, magnesium, total protein, triglycerides; and negatively impacted settings so causing an underestimation of the result: alanine amino- transferase, gamma glutamyl transferase, lipase, alkaline phosphatase, troponin T hypersensitive. Certain parameters influence of hemolysis varies depending on the magnitude of the measured parameter this interference being observed for normal values but disappearing for pathological values: creatinine, cholesterol, alkaline phosphatase, triglycerides, or the inverse interference is greater than for conventional pathological values: lipase, alanine amino-transferase. Knowledge of this variability interference allows the biologist to adapt its methods of reporting in the case of haemolysed samples.
在Cobas 6000 ce分析仪（罗氏诊断公司）上通过实验确定了溶血对22项生化参数的影响。采用添加溶血产物的方法来创建血红蛋白浓度从0到2000 μmol/L逐渐增加的样本。选择10%变化限度来定义溶血对测量结果的影响。所研究的参数分为几类：溶血不影响测量结果的参数：白蛋白、尿酸、钙、C反应蛋白、肌红蛋白、N末端脑钠肽前体、S100蛋白和尿素；溶血对测量结果有正向影响导致结果高估的参数：天冬氨酸氨基转移酶、总胆固醇、肌酸激酶、肌酐、乳酸脱氢酶、镁、总蛋白、甘油三酯；以及溶血对测量结果有负向影响导致结果低估的参数：丙氨酸氨基转移酶、γ-谷氨酰转移酶、脂肪酶、碱性磷酸酶、超敏肌钙蛋白T。某些参数的溶血影响因测量参数的大小而异，这种干扰在正常值时可观察到，但在病理值时消失：肌酐、胆固醇、碱性磷酸酶、甘油三酯；或者反向干扰在病理值时比常规病理值时更大：脂肪酶、丙氨酸氨基转移酶。了解这种变异性干扰可使生物学家在处理溶血样本时调整其报告方法。
