Wolff C, Pepling M, Gergen P, Klingler M
Zoologisches Institut der Universität München, Germany.
Mech Dev. 1999 Jan;80(1):87-99. doi: 10.1016/s0925-4773(98)00196-8.
Pair-rule genes serve two important functions during Drosophila development: they first initiate periodic patterns, and subsequently interact with each other to refine these patterns to the precision required for definition of segmental compartments. Previously, we described a pair-rule input region of the runt gene. Here we further characterize this region through the use of reporter gene constructs and by comparison with corresponding sequences from Drosophila virilis. We find that many but not all regulatory properties of this '7-stripe region' are functionally conserved. Moreover, the similarity between these homologous sequences is surprisingly low. When compared to similar data for gap gene input element, our data suggest that pair-rule target sequences are less constrained during evolution, and that functional elements mediating pair-rule interactions can be dispersed over many kilobases.
它们首先启动周期性模式，随后相互作用以将这些模式细化到定义节段间隔所需的精度。此前，我们描述了runt基因的一个配对规则输入区域。在此，我们通过使用报告基因构建体并与果蝇的相应序列进行比较，进一步对该区域进行了表征。我们发现这个“7条纹区域”的许多但并非所有调控特性在功能上是保守的。此外，这些同源序列之间的相似性出奇地低。与间隙基因输入元件的类似数据相比，我们的数据表明配对规则靶序列在进化过程中受到的限制较少，并且介导配对规则相互作用的功能元件可以分散在许多千碱基上。
