Sikes A, Ehioba R
US Army Soldier System Command, Natick RD and E Center, Attn: SSCNC-WRA, Natick, MA 01760, USA.
Int J Food Microbiol. 1999 Feb 18;46(3):179-85. doi: 10.1016/s0168-1605(98)00179-2.
Previously, it was demonstrated that the combination of sucrose laurate (SL) ethylenediaminetetraacetate (E) and butylated hydroxyl anisole (B) (SLEB) was an effective antimicrobial agent against both gram-negative (aerobes) and gram-positive (facultative anaerobes) foodborne bacteria. This investigation examines the sensitivity of Clostridium perfringens to SLEB relative to: (1) the minimum inhibitory concentration (MIC) of SLEB required to inhibit the growth of C. perfringens and (2) the antibacterial effectiveness of different combination ratios of SLEB in fluid thioglycollate medium (FTM). Results indicated that the MIC of SLEB (1:1:1, v/v/v) against C. perfringens on tryptose sulfite cycloserine (TSC) agar was > 150 ppm at 37 degrees C. However, in FTM, a SLEB (1:1:1, v/v/v) concentration of > 100 ppm inhibited C. perfringens during an incubation (anaerobic) period of 196 h at 37 degrees C. The sensitivity of C. perfringens to different combination ratios was also investigated in FTM. The results showed that, when the concentrations of SL and E were held at 75 ppm in the SLEB combination, and the concentration of B increased from 0 to 75 ppm, C. perfringens growth increased initially during the first 24 h of incubation (37 degrees C) but remained constant during the next 48 h. Similarly, when concentrations of SL and E were held constant at 150 ppm in the SLEB combination and the B ratio increased from 50 to 150 ppm in FTM, C. perfringens viability decreased in all of the treated samples during 72-h incubation at 37 degrees C. The results indicated that SLEB was an effective inhibitor of C. perfringens growth activities, and the ratios of the components of SLEB can be adjusted to meet specific preservation needs.
此前已证明，月桂酸蔗糖酯（SL）、乙二胺四乙酸（E）和丁基化羟基茴香醚（B）的组合（SLEB）是一种针对革兰氏阴性（需氧菌）和革兰氏阳性（兼性厌氧菌）食源细菌的有效抗菌剂。本研究考察了产气荚膜梭菌对SLEB的敏感性，具体涉及：（1）抑制产气荚膜梭菌生长所需的SLEB最低抑菌浓度（MIC），以及（2）在液体硫乙醇酸盐培养基（FTM）中不同组合比例的SLEB的抗菌效果。结果表明，在37℃下，SLEB（1:1:1，v/v/v）对胰蛋白胨亚硫酸盐环丝氨酸（TSC）琼脂上的产气荚膜梭菌的MIC>150 ppm。然而，在FTM中，浓度>100 ppm的SLEB（1:1:1，v/v/v）在37℃下196小时的培养（厌氧）期内抑制了产气荚膜梭菌。还在FTM中研究了产气荚膜梭菌对不同组合比例的敏感性。结果显示，当SLEB组合中SL和E的浓度保持在75 ppm，且B的浓度从0增加到75 ppm时，产气荚膜梭菌在培养（37℃）的最初24小时内生长最初增加，但在接下来的48小时内保持恒定。同样，当SLEB组合中SL和E的浓度在FTM中保持恒定在150 ppm，且B的比例从50 ppm增加到150 ppm时，在37℃下72小时的培养期间，所有处理样品中的产气荚膜梭菌活力均下降。结果表明，SLEB是产气荚膜梭菌生长活性的有效抑制剂，并且可以调整SLEB各成分的比例以满足特定的保存需求。
