Bozdogan B, Berrezouga L, Kuo M S, Yurek D A, Farley K A, Stockman B J, Leclercq R
Service de Bactériologie-Virologie, Hôpital Henri Mondor-Université Paris XII, Créteil, France.
Antimicrob Agents Chemother. 1999 Apr;43(4):925-9. doi: 10.1128/AAC.43.4.925.
Resistance to lincomycin and clindamycin in the clinical isolate Enterococcus faecium HM1025 is due to a ribosomal methylase encoded by an ermAM-like gene and the plasmid-mediated inactivation of these antibiotics. We have cloned and determined the nucleotide sequence of the gene responsible for the inactivation of lincosamides, linB. This gene encodes a 267-amino-acid lincosamide nucleotidyltransferase. The enzyme catalyzes 3(5'-adenylation) (the adenylation of the hydroxyl group in position 3 of the molecules) of lincomycin and clindamycin. Expression of linB was observed in both Escherichia coli and Staphylococcus aureus. The deduced amino acid sequence of the enzyme did not display any significant homology with staphylococcal nucleotidyltransferases encoded by linA and linA' genes. Sequences homologous to linB were found in 14 other clinical isolates of E. faecium, indicating the spread of the resistance trait in this species.
临床分离株屎肠球菌HM1025对林可霉素和克林霉素的耐药性是由于ermAM样基因编码的核糖体甲基化酶以及这些抗生素的质粒介导失活。我们克隆并测定了负责林可酰胺失活的基因linB的核苷酸序列。该基因编码一种267个氨基酸的林可酰胺核苷酸转移酶。该酶催化林可霉素和克林霉素的3(5'-腺苷化)（分子3位羟基的腺苷化）。在大肠杆菌和金黄色葡萄球菌中均观察到linB的表达。该酶推导的氨基酸序列与linA和linA'基因编码的葡萄球菌核苷酸转移酶没有任何显著同源性。在其他14株屎肠球菌临床分离株中发现了与linB同源的序列，表明该耐药性状在该菌种中传播。
