Leclercq R, Brisson-Noël A, Duval J, Courvalin P
Service de Bactériologie-Virologie, Hôpital Henri Mondor, Université Paris XII, Créteil, France.
Antimicrob Agents Chemother. 1987 Dec;31(12):1887-91. doi: 10.1128/AAC.31.12.1887.
We examined the resistance phenotype and the genetic basis of lincosamide modification in 25 clinical isolates of Staphylococcus spp. inactivating lincomycin and clindamycin. The strains were resistant to high levels of lincomycin but remained susceptible to clindamycin. However, MBCs and inoculum effects showed that the activity of clindamycin was impaired. The distribution in these strains of nucleotide sequences related to linA and linA', the genes encoding lincosamide nucleotidylation in Staphylococcus haemolyticus BM4610 and S. aureus BM4611, respectively, was studied by dot blot hybridization. The genes responsible for lincosamide inactivation in Staphylococcus spp. were found to constitute a family of related sequences which are not species specific.
我们检测了25株使林可霉素和克林霉素失活的葡萄球菌属临床分离株的耐药表型及林可酰胺修饰的遗传基础。这些菌株对高水平林可霉素耐药，但对克林霉素仍敏感。然而，最低杀菌浓度（MBC）和接种量效应表明克林霉素的活性受到了损害。通过斑点杂交研究了与linA和linA'相关的核苷酸序列在这些菌株中的分布，linA和linA'分别是编码溶血葡萄球菌BM4610和金黄色葡萄球菌BM4611中林可酰胺核苷酸化的基因。发现葡萄球菌属中负责林可酰胺失活的基因构成了一个相关序列家族，该家族不具有种属特异性。
