Shuvalov L P, Grekova N A, Vershilova P A
Zh Mikrobiol Epidemiol Immunobiol. 1976 Sep(9):59-61.
In studying guinea pigs immunized with brucella protective antigen and the immunological response in them it was found that the antigen could be determined for 30 days by the method of fluorescent antibodies in the cells of the lymph nodes and the spleen following its intravenous injection. The optimal immunizing dose was 0.6 mg; in difference from a dose of 2 mg it produced an earlier and a more pronounced humoral effect. The phagocytic activity of the peritoneal macrophages of guinea pigs immunized with low doses was much more intensive than of those immunized with high doses of the antigen. Brucella protective antigen in a dose of 2 mg produced a disturbance of the carbohydrate metabolism in phagocytes, this influencing negatively their phagocytic function.
在研究用布鲁氏菌保护性抗原免疫豚鼠及其免疫反应时发现，静脉注射该抗原后，采用荧光抗体法可在30天内于淋巴结和脾脏细胞中检测到该抗原。最佳免疫剂量为0.6毫克；与2毫克剂量不同，它产生更早且更明显的体液效应。用低剂量抗原免疫的豚鼠腹腔巨噬细胞的吞噬活性比用高剂量抗原免疫的豚鼠的吞噬活性强得多。2毫克剂量的布鲁氏菌保护性抗原会导致吞噬细胞碳水化合物代谢紊乱，这对其吞噬功能产生负面影响。
