Pizzoferrato A, Ciapetti G, Stea S, Cenni E, Arciola C R, Granchi D, Savarino L
Laboratory for Biocompatibility Research on Implant Materials, Istituti Ortopedici Rizzoli, Bologna, Italy.
Clin Mater. 1994;15(3):173-90. doi: 10.1016/0267-6605(94)90081-7.
Cell culture systems may be of value in testing the biocompatibility of prosthetic materials before they are introduced into clinical use. In recent years, in vitro methods for assaying biomaterials have gained in importance owing to the growing concern over the use of animals for biomaterials testing. Significant effort is therefore being focused toward developing predictive and quantitative, but also simple and reliable, methods of testing using cultured cells. At present, a number of methods for measuring both the cytotoxicity and the specific cytocompatibility of different materials are available. The usefulness of these systems is no longer confined to screening new materials; they can be used to study the mechanisms of action of various materials during tissue/material interaction. This paper reviews the published literature on the use of cell culture models in evaluating biocompatibility and reports on the personal experience of the authors, who have been using cell culture systems for many years and for different purposes.
细胞培养系统在测试假体材料临床应用前的生物相容性方面可能具有重要价值。近年来，由于对使用动物进行生物材料测试的担忧日益增加，体外检测生物材料的方法变得愈发重要。因此，大量工作集中在开发使用培养细胞进行预测性、定量性，同时又简单可靠的测试方法。目前，有多种用于测量不同材料细胞毒性和特定细胞相容性的方法。这些系统的用途不再局限于筛选新材料；它们可用于研究各种材料在组织/材料相互作用过程中的作用机制。本文综述了关于使用细胞培养模型评估生物相容性的已发表文献，并报告了作者的个人经验，作者多年来出于不同目的一直在使用细胞培养系统。
