Sinagowitz E, Pelz K, Burgert A, Kaczkowski W
Infection. 1976;4(4):192-5. doi: 10.1007/BF01638923.
Tissue concentrations of cefazolin were measured in 18 patients undergoing urological operations. Specimens of skeletal muscle and blood were taken simultaneously at predetermined intervals. The concentrations in serum and homogenized skeletal muscle were determined by means of the agar well diffusion method. A comparison of standard curves obtained with phosphate buffer solution and the supernatant of muscle homogenate revealed no substantial binding of cefazolin to the supernatant of muscle homogenate. Because of methodological difficulties in determining the different compartments of a tissue specimen concentrations in tissue were not corrected. Within two hours after a short infusion (25 min.) of 2 g of Cefazolin in muscle tissue a peak concentration of 20 mug/g was reached. A tissue level above 10 mug/g maintained for three hours. This concentration is high enough to inhibit nearly all strains of E. coli, Klebsiella, Salmonella, Shigella and a large part of Proteus mirabilis, whereas nearly all gram-positive bacteria are inhibited at a lower concentration.
对18例接受泌尿外科手术的患者进行了头孢唑林的组织浓度测定。在预定的时间间隔同时采集骨骼肌和血液样本。采用琼脂孔扩散法测定血清和匀浆骨骼肌中的浓度。用磷酸盐缓冲溶液和肌肉匀浆上清液获得的标准曲线比较显示，头孢唑林与肌肉匀浆上清液无明显结合。由于在确定组织标本不同区室时存在方法学困难，未对组织中的浓度进行校正。在肌肉组织中短时间输注（25分钟）2g头孢唑林后两小时内，达到了20μg/g的峰值浓度。组织水平高于10μg/g持续了三小时。该浓度足以抑制几乎所有大肠杆菌、克雷伯菌、沙门菌、志贺菌菌株以及大部分奇异变形杆菌，而几乎所有革兰氏阳性菌在较低浓度下即被抑制。
