Johnson I
Molecular Probes, Eugene, OR 97402-9165, USA.
Histochem J. 1998 Mar;30(3):123-40. doi: 10.1023/a:1003287101868.
The functional characteristics of fluorescent probes used for imaging and measuring dynamic processes in living cells are reviewed. Initial consideration is given to general design requirements for delivery, targeting, detectability and fluorescence readout, and current technologies for attaining them. Discussion then proceeds to the more application-specific properties of intracellular ion indicators, membrane potential sensors, probes for proteins and lipids, and cell viability markers.
本文综述了用于成像和测量活细胞动态过程的荧光探针的功能特性。首先考虑了关于递送、靶向性、可检测性和荧光读数的一般设计要求，以及实现这些要求的当前技术。接着讨论了细胞内离子指示剂、膜电位传感器、蛋白质和脂质探针以及细胞活力标记物等更具应用特异性的特性。
