pH探针的比率成像。

Ratiometric imaging of pH probes.

作者信息

Grillo-Hill Bree K, Webb Bradley A, Barber Diane L

机构信息

Department of Cell and Tissue Biology, University of California, San Francisco, California, USA.

出版信息

Methods Cell Biol. 2014;123:429-48. doi: 10.1016/B978-0-12-420138-5.00023-9.

DOI:10.1016/B978-0-12-420138-5.00023-9

PMID:24974041

Abstract

Measurement of intracellular pH can be readily accomplished using tools and methods described in this chapter. We present a discussion of technical considerations of various ratiometric pH-sensitive probes including dyes and genetically encoded sensors. These probes can be used to measure pH across physical scales from macroscopic whole-mount tissues down to organelles and subcellular domains. We describe protocols for loading pH-sensitive probes into single cells or tissues and discuss ratiometric image acquisition and analysis.

摘要

使用本章所述的工具和方法可以轻松实现细胞内pH值的测量。我们将讨论各种比率型pH敏感探针（包括染料和基因编码传感器）的技术要点。这些探针可用于测量从宏观的整装组织到细胞器和亚细胞区域等不同物理尺度的pH值。我们描述了将pH敏感探针加载到单细胞或组织中的实验方案，并讨论了比率型图像采集和分析。