Newcastle University Bioscience Institute, Cookson Building, Medical School, Newcastle University, Framlington Place, Newcastle-Upon-TyneNE2 4HH, UK.
Animal and Plant Health Agency, Staplake Mount, StarcrossEX6 8PE, UK.
Parasitology. 2021 Sep;148(11):1339-1344. doi: 10.1017/S003118202100086X. Epub 2021 Jun 3.
Tritrichomonas foetus is a venereal trichomonad parasite which causes reproductive issues in cattle. No other trichomonads are known to be urogenital pathogens in cattle, but there are several reports of Tetratrichomonas and Pentatrichomonas isolates of unclear origin from the cattle urogenital tract (UGT) in the Americas. This study reports the first case of a non-T. foetus cattle urogenital trichomonad isolate in Europe. Molecular analysis of the internal transcribed spacer (ITS) 1-5.8S ribosomal RNA-ITS 2 and 18S ribosomal RNA loci suggest that the isolate is a Tetratrichomonas species from a lineage containing other previously described bull preputial isolates. We identified close sequence similarity between published urogenital and gastrointestinal Tetratrichomonas spp., and this is reviewed alongside further evidence regarding the gastrointestinal origin of non-T. foetus isolates. Routine screening for T. foetus is based on culture and identification by microscopy, and so considering other trichomonad parasites of the bovine UGT is important to avoid misdiagnosis.
胎儿三毛滴虫是一种引起牛生殖问题的性病毛滴虫寄生虫。目前还没有其他毛滴虫被认为是牛的尿路病原体，但有几例来自美洲牛泌尿生殖道（UGT）的未明来源的四毛滴虫和五毛滴虫的报道。本研究报告了在欧洲首例非胎儿毛滴虫牛泌尿生殖道毛滴虫分离株的病例。对内转录间隔区（ITS）1-5.8S 核糖体 RNA-ITS 2 和 18S 核糖体 RNA 基因座的分子分析表明，该分离株是来自含有其他先前描述的公牛包皮分离株的四毛滴虫种。我们在已发表的泌尿生殖道和胃肠道四毛滴虫属之间发现了密切的序列相似性，这与关于非胎儿毛滴虫属分离株的胃肠道起源的进一步证据一起进行了审查。基于培养和显微镜鉴定的胎儿三毛滴虫常规筛查，因此考虑牛泌尿生殖道的其他毛滴虫寄生虫很重要，以避免误诊。
