Sukroongreung S, Kitiniyom K, Nilakul C, Tantimavanich S
Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
Med Mycol. 1998 Dec;36(6):419-24.
Basidiospores of Filobasidiella neoformans var. neoformans (progeny of Cryptococcus neoformans MT 100.1 x VR 45980) were able to induce cryptococcosis in Swiss albino mice if inoculated by intraperitoneal injection, nasal instillation or nasal spraying. The latter method, with the aid of a jet nebulizer, was first adopted to imitate the natural entrance of infectious particles. Using this method the small number of basidiospores (7 x 10(3)) could induce cryptococcosis in mice, while the higher number of the parental laboratory-grown yeast cells (1.5 x 10(6)) did not produce infections. By nasal instillation Cyclophosphamide (Cy)-treated mice were more susceptible to the basidiospores, showing 80% cryptococcosis (eight of 10). Seven of the eight infected mice had disseminated cryptococcosis. Immunocompetent mice were more resistant to basidiospore infection than Cy-treated mice, as 40% of that group developed only pulmonary cryptococcosis; none had disseminated infection. Thus, we propose that basidiospores are one form of the infectious propagules of F. neoformans var. neoformans which can cause cryptococcosis, particularly in immunocompromised people.
新型隐球菌新生变种（新型隐球菌MT 100.1×VR 45980的子代）的担孢子若通过腹腔注射、滴鼻或喷雾接种，能够在瑞士白化小鼠中诱发隐球菌病。后一种方法借助喷射雾化器，首次用于模拟感染性颗粒的自然进入途径。使用这种方法，少量担孢子（7×10³）即可在小鼠中诱发隐球菌病，而数量较多的实验室培养亲代酵母细胞（1.5×10⁶）却未引发感染。经滴鼻给予环磷酰胺（Cy）处理的小鼠对担孢子更易感，隐球菌病发生率为80%（10只中有8只）。8只感染小鼠中有7只发生播散性隐球菌病。免疫功能正常的小鼠比经Cy处理的小鼠对担孢子感染更具抵抗力，该组中40%仅发生肺部隐球菌病；无一例发生播散性感染。因此，我们提出担孢子是新型隐球菌新生变种的一种感染性繁殖体形式，可引起隐球菌病，尤其是在免疫功能低下的人群中。
