Flemming R G, Murphy C J, Abrams G A, Goodman S L, Nealey P F
Department of Surgical Sciences, School of Veterinary Medicine, UW-Madison, WI 53706, USA.
Biomaterials. 1999 Mar;20(6):573-88. doi: 10.1016/s0142-9612(98)00209-9.
Topographical cues, independent of biochemistry, generated by the extracellular matrix may have significant effects upon cellular behavior. Studies have documented that substratum topography has direct effects on the ability of cells to orient themselves, migrate, and produce organized cytoskeletal arrangements. Basement membranes are composed of extracellular matrix proteins and found throughout the vertebrate body, serving as substrata for overlying cellular structures. The topography of basement membranes is a complex meshwork of pores, fibers, ridges, and other features of nanometer sized dimensions. Synthetic surfaces with topographical features have been shown to influence cell behavior. These facts lead to the hypothesis that the topography of the basement membrane plays an important role in regulating cellular behavior in a manner distinct from that of the chemistry of the basement membrane. This paper describes the topography of the basement membrane and reviews the fabrication of synthetic micro- and nano-structured surfaces and the effects of such textured surfaces on cell behavior.
由细胞外基质产生的、独立于生物化学的拓扑学线索可能对细胞行为产生重大影响。研究表明，基质拓扑结构对细胞定向、迁移以及产生有组织的细胞骨架排列的能力有直接影响。基底膜由细胞外基质蛋白组成，遍布脊椎动物全身，作为覆盖其上的细胞结构的基质。基底膜的拓扑结构是由孔隙、纤维、脊以及其他纳米尺寸特征构成的复杂网络。具有拓扑特征的合成表面已被证明会影响细胞行为。这些事实引发了这样一种假设，即基底膜的拓扑结构在调节细胞行为方面发挥着重要作用，其方式不同于基底膜的化学性质。本文描述了基底膜的拓扑结构，并综述了合成微纳结构表面的制备以及此类纹理表面对细胞行为的影响。
