Yamada Masahiro, Kato Eiji, Yamamoto Akiko, Sakurai Kaoru
Department of Removable Prosthodontics and Gerodontology, Tokyo Dental College, Tokyo, Japan. Division of Molecular and Regenerative Prosthodontics, Tohoku University Graduate School of Dentistry, Miyagi, Japan.
Biomed Mater. 2016 Feb 2;11(1):015010. doi: 10.1088/1748-6041/11/1/015010.
The acquisition of substantial dermal sealing determines the prognosis of percutaneous titanium-based medical devices or prostheses. A nano-topographic titanium surface with ordered nano-spikes and pores has been shown to induce periodontal-like connective tissue attachment and activate gingival fibroblastic functions. This in vitro study aimed to determine whether an alkali-heat (AH) treatment-created nano-topographic titanium surface could enhance human dermal fibroblastic functions and binding strength to the deposited collagen on the titanium surface. The surface topographies of commercially pure titanium machined discs exposed to two different AH treatments were evaluated. Human dermal fibroblastic cultures grown on the discs were evaluated in terms of cellular morphology, proliferation, extracellular matrix (ECM) and proinflammatory cytokine synthesis, and physicochemical binding strength of surface-deposited collagen. An isotropically-patterned, shaggy nano-topography with a sponge-like inner network and numerous well-organized, anisotropically-patterned fine nano-spikes and pores were observed on each nano-topographic surface type via scanning electron microscopy. In contrast to the typical spindle-shaped cells on the machined surfaces, the isotropically- and anisotropically-patterned nano-topographic titanium surfaces had small circular/angular cells containing contractile ring-like structures and elongated, multi-shaped cells with a developed cytoskeletal network and multiple filopodia and lamellipodia, respectively. These nano-topographic surfaces enhanced dermal-related ECM synthesis at both the protein and gene levels, without proinflammatory cytokine synthesis or reduced proliferative activity. Deposited collagen fibers were included in these surfaces and sufficiently bound to the nano-topographies to resist the physical, enzymatic and chemical detachment treatments, in contrast to machined surfaces. Well-organized, isotropically-/anisotropically-patterned, nano-topographic titanium surfaces with AH treatment-created nano-spikes and pores enhanced human dermal fibroblastic ECM synthesis and established sufficient mechanical integration between the surfaces and ECM to resist various detachment treatments used to experimentally mimic overloading and inflammation.
大量真皮密封的形成决定了经皮钛基医疗器械或假体的预后。具有有序纳米尖峰和孔隙的纳米拓扑钛表面已被证明可诱导牙周样结缔组织附着并激活牙龈成纤维细胞功能。本体外研究旨在确定碱热（AH）处理产生的纳米拓扑钛表面是否能增强人真皮成纤维细胞功能以及与钛表面沉积胶原蛋白的结合强度。对经过两种不同AH处理的商业纯钛加工圆盘的表面形貌进行了评估。对在圆盘上生长的人真皮成纤维细胞培养物进行了细胞形态、增殖、细胞外基质（ECM）和促炎细胞因子合成以及表面沉积胶原蛋白的物理化学结合强度方面的评估。通过扫描电子显微镜在每种纳米拓扑表面类型上观察到具有海绵状内部网络以及众多组织良好、各向异性排列的精细纳米尖峰和孔隙的各向同性图案的蓬松纳米拓扑。与加工表面上典型的纺锤形细胞相比，各向同性和各向异性图案的纳米拓扑钛表面分别具有包含收缩环状结构的小圆形/角形细胞以及具有发达细胞骨架网络和多个丝状伪足和片状伪足的细长、多形状细胞。这些纳米拓扑表面在蛋白质和基因水平上均增强了与真皮相关的ECM合成，且无促炎细胞因子合成或增殖活性降低。与加工表面相比，沉积的胶原纤维包含在这些表面中并与纳米拓扑充分结合，以抵抗物理、酶促和化学分离处理。经过AH处理产生纳米尖峰和孔隙的组织良好、各向同性/各向异性图案的纳米拓扑钛表面增强了人真皮成纤维细胞ECM合成，并在表面与ECM之间建立了足够的机械整合，以抵抗用于实验模拟过载和炎症的各种分离处理。
