Flechner A, Gross W, Martin W F, Schnarrenberger C
Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität Berlin, Germany.
FEBS Lett. 1999 Mar 26;447(2-3):200-2. doi: 10.1016/s0014-5793(99)00285-9.
Class I and class II aldolases are products of two evolutionary non-related gene families. The cytosol and chloroplast enzymes of higher plants are of the class I type, the latter being bifunctional for fructose-1,6- and sedoheptulose-1,7-P2 in the Calvin cycle. Recently, class II aldolases were detected for the cytosol and chloroplasts of the lower alga Cyanophora paradoxa. The respective chloroplast enzyme has been shown here to be also bifunctional for fructose-1,6- and sedoheptulose-1,7-P2. Kinetics, also including fructose-1-P, were determined for all these enzymes. Apparently, aldolases are multifunctional enzymes, irrespective of their class I or class II type.
I类和II类醛缩酶是两个进化上不相关的基因家族的产物。高等植物的胞质溶胶和叶绿体酶属于I类，后者在卡尔文循环中对果糖-1,6-二磷酸和景天庚酮糖-1,7-二磷酸具有双功能。最近，在低等藻类蓝氏拟甲色球藻的胞质溶胶和叶绿体中检测到了II类醛缩酶。这里已表明相应的叶绿体酶对果糖-1,6-二磷酸和景天庚酮糖-1,7-二磷酸也具有双功能。测定了所有这些酶的动力学，也包括果糖-1-磷酸的动力学。显然，醛缩酶是多功能酶，无论其是I类还是II类。
