Tani N, Watanabe Y, Suzuki T, Muramatsu S, Miyazawa M, Kimura N, Miwa T
Department of Internal Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Dig Dis Sci. 1999 Apr;44(4):686-90. doi: 10.1023/a:1026645321698.
The effects of inflammatory cytokines induced by Helicobacter pylori infection on acid secretion have not been well defined. The purpose of this study was to investigate the direct effects of these cytokines on parietal cells isolated from guinea pigs. We examined the effects of human recombinant IL-1beta (0.05-100 ng/ml), IL-8 (2-256 ng/ml), and TNF-alpha (0.625-80 ng/ml) on acid secretion stimulated by three secretagogues (10(-4) M histamine, 10(-4) M carbachol, and 10(-5) M tetragastrin) and on basal acid secretion from isolated parietal cells, which was measured by the aminopyrine accumulation method. None of three cytokines showed any significant effects on stimulated or basal acid secretion from isolated guinea pig parietal cells. We concluded that inflammatory cytokines induced by Helicobacter pylori infection may affect acid secretion through mechanisms other than direct actions on parietal cells.
幽门螺杆菌感染诱导的炎性细胞因子对胃酸分泌的影响尚未明确界定。本研究的目的是调查这些细胞因子对从豚鼠分离出的壁细胞的直接作用。我们检测了人重组白细胞介素-1β（0.05 - 100纳克/毫升）、白细胞介素-8（2 - 256纳克/毫升）和肿瘤坏死因子-α（0.625 - 80纳克/毫升）对三种促分泌剂（10⁻⁴摩尔/升组胺、10⁻⁴摩尔/升卡巴胆碱和10⁻⁵摩尔/升五肽胃泌素）刺激的胃酸分泌以及对通过氨基比林蓄积法测量的分离壁细胞基础胃酸分泌的影响。三种细胞因子均未对分离的豚鼠壁细胞的刺激胃酸分泌或基础胃酸分泌显示出任何显著影响。我们得出结论，幽门螺杆菌感染诱导的炎性细胞因子可能通过对壁细胞的直接作用以外的机制影响胃酸分泌。
