Lee A J, Walsh T F, Hodges S J, Rawlinson A
Department of Restorative Dentistry, School of Clinical Dentistry, University of Sheffield, UK.
J Clin Periodontol. 1999 Apr;26(4):252-6. doi: 10.1034/j.1600-051x.1999.260409.x.
This study aimed to detect the levels of osteocalcin in gingival crevicular fluid (GCF) from healthy (< or =3 mm sulcus depth and non-bleeding) and diseased sites (> or =6 mm probing depth and bleeding) in subjects with adult periodontitis, in order to further investigate its potential as a possible marker of the disease process. Periodontal probing depths, attachment levels and gingival indices were recorded from one healthy and one diseased site in each of 20 subjects with adult periodontitis. Both GCF accumulated in the periodontal pocket or sulci and GCF flowing into the periodontal pocket or sulci over a three-minute interval were sampled. The amounts of osteocalcin in each GCF sample was determined using immunoassays. A mean of 2.34 ng/site (2.7 microg/ml) osteocalcin was found at diseased sites and a mean of 2.47 ng/site (5.47 microg/ml) was found at healthy sites for the accumulated GCF collection method. A mean of 0.17 ng/ site (2.17 microg/ml) osteocalcin was found at diseased sites and a mean of 0.14 ng/ site (1.85 microg/ml) at healthy sites for the flow method of GCF collection. There were no statistically significant differences between osteocalcin levels in diseased and healthy sites in subjects with adult periodontitis.
本研究旨在检测成人牙周炎患者健康部位（龈沟深度≤3mm且不出血）和病变部位（探诊深度≥6mm且出血）龈沟液（GCF）中骨钙素的水平，以进一步研究其作为疾病进程潜在标志物的可能性。记录了20例成人牙周炎患者每个患者一个健康部位和一个病变部位的牙周探诊深度、附着水平和牙龈指数。采集了在牙周袋或龈沟中积聚的GCF以及在三分钟内流入牙周袋或龈沟的GCF。使用免疫测定法测定每个GCF样本中骨钙素的含量。对于积聚GCF的采集方法，病变部位骨钙素的平均含量为2.34ng/部位（2.7μg/ml），健康部位为2.47ng/部位（5.47μg/ml）。对于GCF流动采集方法，病变部位骨钙素的平均含量为0.17ng/部位（2.17μg/ml），健康部位为0.14ng/部位（1.85μg/ml）。成人牙周炎患者病变部位和健康部位的骨钙素水平之间无统计学显著差异。
