Linden G J, McKinnell J, Shaw C, Lundy F T
Division of Restorative Dentistry (Periodontics), School of Clinical Dentistry, Queen's University, Northern Ireland, UK.
J Clin Periodontol. 1997 Nov;24(11):799-803. doi: 10.1111/j.1600-051x.1997.tb01192.x.
The aims of the present study were to investigate whether the tachykinins substance P and neurokinin A were present in gingival crevicular fluid in both periodontal health and disease and to study the relationship with periodontal inflammation. Gingival crevicular fluid (GCF) was collected from a healthy, a gingivitis and a periodontitis site in 20 subjects with periodontitis and from a healthy site in 20 subjects without periodontitis. The volume of GCF was measured and each sample subsequently analysed for substance P and neurokinin A by radioimmunoassay. There were significantly increased levels of substance P-like immunoreactivity (SP-LI) and neurokinin A-like immunoreactivity (NKA-LI) in gingivitis and periodontitis sites compared with healthy sites. Both tachykinins were significantly elevated in periodontitis affected subjects, with significantly more tachykinin-like immunoreactivity at healthy sites in periodontitis affected compared with periodontally-healthy subjects. Despite the considerable individual variation in the levels of SP-LI and NKA-LI, both tachykinins were present at levels at which they could have biological activity. It is concluded that substance P and neurokinin A may have a rôle in the pathogenesis of periodontal disease and that further investigations could prove useful in clarifying the mechanisms through which neuropeptides could modulate periodontal health and disease.
本研究的目的是调查速激肽P物质和神经激肽A在牙周健康和疾病状态下的龈沟液中是否存在，并研究其与牙周炎症的关系。从20名患有牙周炎的受试者的健康部位、牙龈炎部位和牙周炎部位以及20名未患牙周炎的受试者的健康部位收集龈沟液（GCF）。测量GCF的体积，随后通过放射免疫分析法对每个样本进行P物质和神经激肽A的分析。与健康部位相比，牙龈炎和牙周炎部位的P物质样免疫反应性（SP-LI）和神经激肽A样免疫反应性（NKA-LI）水平显著升高。在患有牙周炎的受试者中，两种速激肽均显著升高，与牙周健康的受试者相比，患有牙周炎的受试者在健康部位的速激肽样免疫反应性明显更高。尽管SP-LI和NKA-LI水平存在相当大的个体差异，但两种速激肽的水平都可能具有生物活性。得出的结论是，P物质和神经激肽A可能在牙周疾病的发病机制中起作用，进一步的研究可能有助于阐明神经肽调节牙周健康和疾病的机制。
