Shazam Hamda, Shaikh Fouzia, Hussain Zaheer, Majeed M Mansoor, Khan Saba, Khurshid Zohaib
Department of Oral Pathology, College of Dentistry, Ziauddin University, Karachi, Pakistan.
Department of Pathology, Ziauddin University, Karachi, Pakistan.
Eur J Dent. 2020 Jul;14(3):352-359. doi: 10.1055/s-0040-1710143. Epub 2020 May 21.
The present study aimed to investigate osteocalcin levels in saliva of healthy and periodontitis patients and correlate these levels with periodontitis severity.
This cross-sectional study was conducted in a hospital setup. A total of 95 individuals participated in the study with 46 subjects in group I (healthy individuals) and 49 subjects in group II (mild, moderate, and severe chronic periodontitis patients). A detailed assessment of clinical periodontal parameters and alveolar bone loss was made. Unstimulated saliva samples were collected from all study subjects and osteocalcin levels were quantitatively analyzed by sandwich enzyme-linked immunosorbent essay technique.
One-way analysis of variance, Spearman's correlation test, and Pearson's chi-squared test were applied at a significance level of 95%. -Values less than 0.05 were considered statistically significant.
The results showed a significant association of qualification with group II ( < 0.02). Bone loss scores were also significantly associated with periodontitis severity ( < 0.01). However, no statistically significant difference was observed between group I and group II in terms of mean salivary osteocalcin levels ( = 0.68). Also, an insignificant correlation was also observed between osteocalcin levels and periodontitis severity ( = 0.13).
The overall study results showed that there was no significant difference between saliva osteocalcin levels of healthy and periodontitis patients. Also, there was a nonsignificant correlation between osteocalcin levels and periodontitis severity. The findings of the present study support the hypothesis that low osteocalcin levels in saliva might be considered as a poor indicator of periodontal disease progression and severity.
本研究旨在调查健康人和牙周炎患者唾液中的骨钙素水平，并将这些水平与牙周炎严重程度相关联。
本横断面研究在医院环境中进行。共有95人参与研究，其中第一组46名受试者（健康个体），第二组49名受试者（轻度、中度和重度慢性牙周炎患者）。对临床牙周参数和牙槽骨丧失进行了详细评估。收集所有研究对象的非刺激性唾液样本，并采用夹心酶联免疫吸附试验技术对骨钙素水平进行定量分析。
采用单因素方差分析、Spearman相关检验和Pearson卡方检验，显著性水平为95%。P值小于0.05被认为具有统计学意义。
结果显示，第二组的相关因素具有显著相关性（P<0.02）。骨丧失评分也与牙周炎严重程度显著相关（P<0.01）。然而，第一组和第二组之间在唾液骨钙素平均水平方面未观察到统计学显著差异（P = 0.68）。此外，骨钙素水平与牙周炎严重程度之间也观察到不显著的相关性（P = 0.13）。
总体研究结果表明，健康人和牙周炎患者的唾液骨钙素水平之间没有显著差异。此外，骨钙素水平与牙周炎严重程度之间存在不显著的相关性。本研究结果支持以下假设：唾液中骨钙素水平低可能被视为牙周疾病进展和严重程度的不良指标。
