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酿酒酵母ATH1基因缺失突变体在葡萄糖上的生长增强是突变株和参照株营养缺陷型不匹配造成的假象。

Elevated growth of Saccharomyces cerevisiae ATH1 null mutants on glucose is an artifact of nonmatching auxotrophies of mutant and reference strains.

作者信息

Chopra R, Sharma V M, Ganesan K

机构信息

Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.

出版信息

Appl Environ Microbiol. 1999 May;65(5):2267-8. doi: 10.1128/AEM.65.5.2267-2268.1999.

DOI:10.1128/AEM.65.5.2267-2268.1999
PMID:10224035
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC91332/
Abstract

Yeast strains disrupted for ATH1, which encodes vacuolar acid trehalase, have been reported to grow to higher cell densities than reference strains. We showed that the increase in cell density is due to the URA3 gene introduced as a part of the disruption and concluded that the misinterpretation is a result of not using a control strain with matching auxotrophic markers.

摘要

据报道，编码液泡酸性海藻糖酶的ATH1基因被破坏的酵母菌株比对照菌株能生长到更高的细胞密度。我们发现细胞密度的增加是由于作为破坏的一部分引入的URA3基因，并得出结论，这种错误解读是未使用具有匹配营养缺陷型标记的对照菌株的结果。

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Elevated growth of Saccharomyces cerevisiae ATH1 null mutants on glucose is an artifact of nonmatching auxotrophies of mutant and reference strains.酿酒酵母ATH1基因缺失突变体在葡萄糖上的生长增强是突变株和参照株营养缺陷型不匹配造成的假象。
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本文引用的文献

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Pyruvate metabolism in Saccharomyces cerevisiae.酿酒酵母中的丙酮酸代谢
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Deletion of the ATH1 gene in Saccharomyces cerevisiae prevents growth on trehalose.酿酒酵母中ATH1基因的缺失会阻止其在海藻糖上生长。
FEBS Lett. 1996 May 20;386(2-3):235-8. doi: 10.1016/0014-5793(96)00450-4.
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Disruption of the yeast ATH1 gene confers better survival after dehydration, freezing, and ethanol shock: potential commercial applications.酵母ATH1基因的破坏使酵母在脱水、冷冻和乙醇冲击后具有更好的存活率：潜在的商业应用。
Appl Environ Microbiol. 1996 May;62(5):1563-9. doi: 10.1128/aem.62.5.1563-1569.1996.
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Yeast. 1995 Sep 15;11(11):1015-25. doi: 10.1002/yea.320111103.
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