Jeanthon C, L'Haridon S, Pradel N, Prieur D
Station Biologique, UPR9042, Centre National de la Recherche Scientifique, Roscoff, France.
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:591-4. doi: 10.1099/00207713-49-2-591.
16S rDNAs amplified by PCR from 22 hyperthermophilic methanococci isolated from deep-sea hydrothermal vents were compared with those of the six type strains of the genus Methanococcus by RFLP analysis. Restriction fragments obtained with Haelli enabied four of the type species to be distinguished. Restrictions with HhaI, BstUI and MspI were necessary to differentiate Methanococcus jannaschii and Methanococcus fervens. The results indicate that the 16S rDNA PCR-RFLP method provides a rapid and reliable tool for the identification of newly isolated hyperthermophilic Methanococcus spp.
通过聚合酶链式反应（PCR）从22株分离自深海热液喷口的嗜热甲烷球菌中扩增出16S核糖体DNA（rDNA），并通过限制性片段长度多态性分析（RFLP）将其与甲烷球菌属的6株模式菌株的16S rDNA进行比较。用HaeIII获得的限制性片段可区分4个模式种。为了区分詹氏甲烷球菌和嗜热甲烷球菌，需要用HhaI、BstUI和MspI进行酶切。结果表明，16S rDNA PCR-RFLP方法为新分离的嗜热甲烷球菌属菌株的鉴定提供了一种快速可靠的工具。
