Ludwig W, Mittenhuber G, Friedrich C G
Lehrstuhl für Mikrobiologie, Technische Universität München, Germany.
Int J Syst Bacteriol. 1993 Apr;43(2):363-7. doi: 10.1099/00207713-43-2-363.
Comparative sequence analysis of in vitro-amplified 16S rRNA genes of Thiosphaera pantotropha GB17T (T = type strain) and Paracoccus denitrificans LMG 4218T revealed identical 16S rRNA primary structures for the two organisms. The level of overall DNA similarity of Thiosphaera pantotropha GB17T and P. denitrificans DSM 65T is 85%, as determined by quantitative DNA-DNA hybridization. Therefore, we propose the transfer of Thiosphaera pantotropha to P. denitrificans. The closest relative of Thiosphaera pantotropha and P. denitrificans is Thiobacillus versutus, as revealed by comparative 16S rRNA sequence analysis. These organisms are members of the alpha subclass of the Proteobacteria. Within this subclass, Thiosphaera pantotropha, P. denitrificans, and Thiobacillus versutus form a phylogenetic group with Rhodobacter sphaeroides, Rhodobacter capsulatus, and "Erythrobacter longus."
嗜油硫杆菌GB17T（T = 模式菌株）和反硝化副球菌LMG 4218T体外扩增的16S rRNA基因的比较序列分析显示，这两种微生物的16S rRNA一级结构相同。通过定量DNA-DNA杂交测定，嗜油硫杆菌GB17T和反硝化副球菌DSM 65T的总体DNA相似性水平为85%。因此，我们建议将嗜油硫杆菌归入反硝化副球菌。比较16S rRNA序列分析表明，嗜油硫杆菌和反硝化副球菌最亲近的亲缘菌是敏捷硫杆菌。这些微生物是变形菌纲α亚纲的成员。在该亚纲内，嗜油硫杆菌、反硝化副球菌和敏捷硫杆菌与球形红杆菌、荚膜红杆菌以及“长赤杆菌”形成一个系统发育类群。
