Tjiong M Y, van der Vange N, ten Kate F J, Tjong-A-Hung S P, ter Schegget J, Burger M P, Out T A
Department of Obstetrics and Gynecology, Academic Medical Center, Amsterdam, The Netherlands.
Gynecol Oncol. 1999 May;73(2):285-91. doi: 10.1006/gyno.1999.5358.
Conflicting data exist on IL-6 production by human papillomavirus (HPV) immortalized cell lines and several cervical carcinoma cell lines. However, no information has been reported on the levels of cytokines in cervicovaginal washings in relation to cervical neoplasia. The aim of this study was to investigate whether local production of IL-6 could be found and whether the level of this cytokine was related to the severity of cervical neoplasia. IL-8 was measured to obtain additional information on an inflammatory cytokine with possible epithelial origin.
Cervicovaginal washings and sera were obtained from 35 patients with invasive cervical cancer, 62 patients with cervical intraepithelial neoplasia (CIN), and 25 control subjects. IL-6 and IL-8 levels were determined by ELISA. HPV DNA in cervical smears was detected by a HPV-16-specific PCR method and additionally by CPI/IIG PCR. Histological analysis of the inflammatory infiltrate was performed on hematoxylin-eosin-stained tissue sections.
In the patients with cervical cancer, those with CIN, and the controls, the median IL-6 concentration in cervicovaginal washings was 171 pg/ml (interquartile range: 54-780), 22 pg/ml (<2-73), and < 2 pg/ml (<2-<2), respectively. For IL-8, the levels were 2756 pg/ml (1651-7107), 489 pg/ml (248-1158), and 631 pg/ml (346-897), respectively. In most subjects the local levels were much higher than in serum. Local IL-6 and IL-8 levels were significantly higher in patients with cervical carcinoma compared with CIN patients and controls. Likewise, local IL-6 levels were increased in patients with CIN compared with controls. No relation was found between cytokine levels and CIN grade or between cytokine levels and the inflammatory infiltrate scored by histological analysis.
There is local production of IL-6 and IL-8 in cervicovaginal secretions, and the production of IL-6 was related to the severity of cervical neoplasia.
关于人乳头瘤病毒（HPV）永生化细胞系和几种子宫颈癌细胞系产生白细胞介素-6（IL-6）的数据存在冲突。然而，尚无关于宫颈阴道灌洗液中细胞因子水平与宫颈肿瘤形成关系的报道。本研究的目的是调查是否能发现局部产生的IL-6，以及该细胞因子的水平是否与宫颈肿瘤形成的严重程度相关。检测IL-8以获取有关可能起源于上皮的炎性细胞因子的更多信息。
从35例浸润性宫颈癌患者、62例宫颈上皮内瘤变（CIN）患者和25例对照受试者中获取宫颈阴道灌洗液和血清。通过酶联免疫吸附测定（ELISA）法测定IL-6和IL-8水平。通过HPV-16特异性聚合酶链反应（PCR）方法并另外通过CPI/IIG PCR检测宫颈涂片中的HPV DNA。对苏木精-伊红染色的组织切片进行炎性浸润的组织学分析。
在宫颈癌患者、CIN患者和对照受试者中，宫颈阴道灌洗液中IL-6的中位浓度分别为171 pg/ml（四分位间距：54 - 780）、22 pg/ml（<2 - 73）和<2 pg/ml（<2 - <2）。对于IL-8，水平分别为2756 pg/ml（1651 - 7107）、489 pg/ml（248 - 1158）和631 pg/ml（346 - 897）。在大多数受试者中，局部水平远高于血清水平。与CIN患者和对照相比，宫颈癌患者的局部IL-6和IL-8水平显著更高。同样，与对照相比，CIN患者的局部IL-6水平升高。未发现细胞因子水平与CIN分级之间或细胞因子水平与组织学分析评分的炎性浸润之间存在关联。
宫颈阴道分泌物中存在局部产生的IL-6和IL-8，且IL-6的产生与宫颈肿瘤形成的严重程度相关。
