Harper R A
Prostaglandins. 1976 Dec;12(6):1019-25. doi: 10.1016/0090-6980(76)90135-0.
Prostaglandins A2, B1, E1, E2, F1alpha and F2alpha were added to cultures of human epidermal cells (keratinocytes) for 24 hours at 37 degrees C, and the effects on 3H-thymidine uptake into DNA was measured. At 70 mu/ml all prostaglandins tested except PGF2alpha inhibited the uptake of 3-thymidine greater than 50%. However, at 35 mug/ml, PGA2 and PGB1 were the only two prostaglandins to show significant inhibition, 96% and 51% respectively. At 17.5 mug/ml only PGA2 caused substantial inhibition, 68%. In order to determine if the PGA2 action was mediated by membrane receptors propranolol, phentolamine, metiamide and prostynoic acid were added in conjunction with PGA2. None of the above receptor antagonists were able to reduce the PGA2-induced inhibition of 3H-thymidine uptake. These results indicate that the pre-incubation of human keratinocytes with prostaglandins for 24 hours results in a decrease of 3H-thymidine incorporation in DNA. The precise mechanism of action is unknown at this time.
将前列腺素A2、B1、E1、E2、F1α和F2α添加到人类表皮细胞（角质形成细胞）培养物中，于37℃孵育24小时，然后检测其对3H-胸腺嘧啶核苷掺入DNA的影响。除PGF2α外，所有测试的前列腺素在浓度为70μg/ml时，对3H-胸腺嘧啶核苷摄取的抑制率均超过50%。然而，在35μg/ml时，仅PGA2和PGB1表现出显著抑制作用，抑制率分别为96%和51%。在17.5μg/ml时，只有PGA2产生了显著抑制作用，抑制率为68%。为了确定PGA2的作用是否通过膜受体介导，将普萘洛尔、酚妥拉明、甲硫咪胺和炔前列酸与PGA2联合添加。上述受体拮抗剂均不能降低PGA2诱导的对3H-胸腺嘧啶核苷摄取的抑制作用。这些结果表明，人角质形成细胞与前列腺素预孵育24小时会导致3H-胸腺嘧啶核苷掺入DNA减少。目前其确切作用机制尚不清楚。
