Hazenberg B P, Limburg P C, Bijzet J, van Rijswijk M H
Department of Medicine, University Hospital, Groningen, The Netherlands.
Ann Rheum Dis. 1999 Feb;58(2):96-102. doi: 10.1136/ard.58.2.96.
To describe a new, quantitative, and reproducible method for detecting deposits of amyloid A protein in aspirated fat tissue and to compare it with smears stained with Congo red.
After extraction of at least 30 mg of abdominal fat tissue in guanidine, the amyloid A protein concentration was measured by a monoclonal antibody-based sandwich ELISA.
The concentrations in 24 patients with arthritis and AA amyloidosis (median 236, range 1.1-8530 ng/mg tissue) were higher (p < 0.001) than in non-arthritic controls, uncomplicated rheumatoid arthritis, and other types of systemic amyloidosis (median 1.1, range 1.1-11.6 ng/mg tissue). Patients with extensive deposits, according to Congo red staining, had higher concentrations than patients with minute deposits.
This is a new, quantitative, and reproducible method for detecting deposits of amyloid A protein in aspirated fat tissue of patients with arthritis, even when minute deposits are present as detected in smears stained with Congo red.
描述一种用于检测抽吸脂肪组织中淀粉样蛋白A沉积的新的、定量且可重复的方法,并将其与刚果红染色涂片进行比较。
在胍中提取至少30mg腹部脂肪组织后,通过基于单克隆抗体的夹心酶联免疫吸附测定法测量淀粉样蛋白A的浓度。
24例患有关节炎和AA淀粉样变性的患者(中位数为236,范围为1.1 - 8530ng/mg组织)的浓度高于非关节炎对照组、单纯类风湿性关节炎患者以及其他类型的系统性淀粉样变性患者(中位数为1.1,范围为1.1 - 11.6ng/mg组织)(p < 0.001)。根据刚果红染色,有大量沉积物的患者浓度高于有微小沉积物的患者。
这是一种用于检测关节炎患者抽吸脂肪组织中淀粉样蛋白A沉积的新的、定量且可重复的方法,即使在刚果红染色涂片中检测到微小沉积物时也是如此。