凝血酶受体介导的信号通过滑膜成纤维细胞中NF-κB的激活诱导白细胞介素6和粒细胞集落刺激因子的表达。
Thrombin receptor mediated signals induce expressions of interleukin 6 and granulocyte colony stimulating factor via NF-kappa B activation in synovial fibroblasts.
作者信息
Shin H, Kitajima I, Nakajima T, Shao Q, Tokioka T, Takasaki I, Hanyu N, Kubo T, Maruyama I
机构信息
Drug Discovery Research Laboratories, Kaken Pharmaceutical Co, Ltd, Kyoto, Japan.
出版信息
Ann Rheum Dis. 1999 Jan;58(1):55-60. doi: 10.1136/ard.58.1.55.
Abstract
OBJECTIVE
To clarify the mechanism of thrombin receptor mediated signal transduction and the induction of cytokines by thrombin stimulation in rheumatoid synovial fibroblasts.
METHODS
Cytokines were measured by enzyme linked immunosorbent assay (ELISA) in the supernatants of cultured rheumatoid synovial fibroblasts stimulated by thrombin. To assess the mechanism of thrombin receptor mediated signal transduction in the rheumatoid synovial fibroblasts, electrophoretic mobility gel shift assay (EMSA), immunoglobulin kappa-chloramphenicol acetyltransferase (CAT) assay, and immunostaining for NF-kappa B subunit molecule was performed.
RESULTS
Thrombin stimulation activated the inducible transcription factor NF-kappa B, and then induced subsequent expressions of interleukin 6 (IL6) and granulocyte colony stimulating factor (G-CSF) in the cells.
CONCLUSION
Thrombin receptor mediated signal transduction could induce the expressions of IL6 and G-CSF, and increase inflammatory events in the cavum articulare via NF-kappa B activation.
摘要
目的
阐明类风湿性滑膜成纤维细胞中凝血酶受体介导的信号转导机制以及凝血酶刺激诱导细胞因子产生的机制。
方法
通过酶联免疫吸附测定（ELISA）检测凝血酶刺激培养的类风湿性滑膜成纤维细胞上清液中的细胞因子。为评估类风湿性滑膜成纤维细胞中凝血酶受体介导的信号转导机制，进行了电泳迁移率凝胶阻滞分析（EMSA）、免疫球蛋白κ-氯霉素乙酰转移酶（CAT）分析以及NF-κB亚基分子的免疫染色。
结果
凝血酶刺激激活了诱导型转录因子NF-κB，随后诱导细胞中白细胞介素6（IL6）和粒细胞集落刺激因子（G-CSF）的后续表达。
结论
凝血酶受体介导的信号转导可通过激活NF-κB诱导IL6和G-CSF的表达，并增加关节腔中的炎症反应。
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