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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biales B, Dichter M, Tischler A

J Physiol. 1976 Nov;262(3):743-53. doi: 10.1113/jphysiol.1976.sp011618.

Adult human and gerbil adrenal medullary cells were maintained in dissociated cell culture and studied by micro-electrode penetration. 2. In the best recordings, chromaffin cell transmembrane potentials exceeded -50mV. 3. Chromaffin cells were capable of generating all-or-nothing over-shooting action potentials, similar to those generated by sympathetic neurones. 4. The action potentials were blocked by tetrodotoxin (TTX, 10(-6)g/ml.) but were not blocked by removal of Ca or by CoCl2 (10 mM). We conclude that the action potentials are probably generated by a Na mechanism. 5. Chromaffin cells are depolarized by the iontophoretic application of acetylcholine (ACh). This depolarization was accompanied by an increased membrane conductance and could trigger action potentials. 6. Action potentials were also found in cells in fresh slices of gerbil adrenal medullae.

将成年人类和沙鼠的肾上腺髓质细胞进行解离细胞培养，并用微电极穿刺法进行研究。2. 在最佳记录中，嗜铬细胞的跨膜电位超过 -50mV。3. 嗜铬细胞能够产生全或无的超射动作电位，类似于交感神经元产生的动作电位。4. 动作电位被河豚毒素（TTX，10(-6)g/ml）阻断，但去除 Ca 或加入 CoCl2（10 mM）并未阻断。我们得出结论，动作电位可能是由 Na 机制产生的。5. 通过离子电泳施加乙酰胆碱（ACh）可使嗜铬细胞去极化。这种去极化伴随着膜电导增加，并可触发动作电位。6. 在沙鼠肾上腺髓质新鲜切片的细胞中也发现了动作电位。

Biales B, Dichter M, Tischler A

J Physiol. 1976 Nov;262(3):743-53. doi: 10.1113/jphysiol.1976.sp011618.

Adult human and gerbil adrenal medullary cells were maintained in dissociated cell culture and studied by micro-electrode penetration. 2. In the best recordings, chromaffin cell transmembrane potentials exceeded -50mV. 3. Chromaffin cells were capable of generating all-or-nothing over-shooting action potentials, similar to those generated by sympathetic neurones. 4. The action potentials were blocked by tetrodotoxin (TTX, 10(-6)g/ml.) but were not blocked by removal of Ca or by CoCl2 (10 mM). We conclude that the action potentials are probably generated by a Na mechanism. 5. Chromaffin cells are depolarized by the iontophoretic application of acetylcholine (ACh). This depolarization was accompanied by an increased membrane conductance and could trigger action potentials. 6. Action potentials were also found in cells in fresh slices of gerbil adrenal medullae.

将成年人类和沙鼠的肾上腺髓质细胞进行解离细胞培养，并用微电极穿刺法进行研究。2. 在最佳记录中，嗜铬细胞的跨膜电位超过 -50mV。3. 嗜铬细胞能够产生全或无的超射动作电位，类似于交感神经元产生的动作电位。4. 动作电位被河豚毒素（TTX，10(-6)g/ml）阻断，但去除 Ca 或加入 CoCl2（10 mM）并未阻断。我们得出结论，动作电位可能是由 Na 机制产生的。5. 通过离子电泳施加乙酰胆碱（ACh）可使嗜铬细胞去极化。这种去极化伴随着膜电导增加，并可触发动作电位。6. 在沙鼠肾上腺髓质新鲜切片的细胞中也发现了动作电位。