van der Wouden E J, de Jong A, Thijs J C, Kleibeuker J H, van Zwet A A
Department of Internal Medicine, Bethesda Hospital, Hoogeveen, The Netherlands.
Antimicrob Agents Chemother. 1999 Jun;43(6):1484-6. doi: 10.1128/AAC.43.6.1484.
Metronidazole susceptibility testing by E test was compared to that by disk diffusion for 263 Helicobacter pylori isolates and to that by breakpoint agar dilution for 90 H. pylori isolates. In 5% and 6% of the cases, respectively, results were discrepant. For each of 52 clinical isolates an E test was performed on 10 separate colonies. Subpopulations of resistant and susceptible bacteria were found in five cases. From three isolates, each colony was subcultured and tested up to 10 times. All but 1 of 292 tests showed the same result. We conclude that the E test is reliable and that subpopulations are responsible for discordant results.
将263株幽门螺杆菌分离株的甲硝唑药敏试验E试验结果与纸片扩散法结果进行比较，并将90株幽门螺杆菌分离株的结果与断点琼脂稀释法结果进行比较。结果分别在5%和6%的病例中出现差异。对52株临床分离株中的每一株，在10个独立菌落上进行E试验。在5例中发现了耐药菌和敏感菌的亚群。从3株分离株中，将每个菌落进行传代培养并测试多达10次。292次试验中除1次外，其余结果均相同。我们得出结论，E试验是可靠的，亚群是导致结果不一致的原因。
