Institute of Medical Research, College of Medicine, Dongguk University-Seoul, Goyang 410-773, Republic of Korea ; College of Pharmacy, Dongguk University-Seoul, Goyang 410-820, Republic of Korea.
Evid Based Complement Alternat Med. 2013;2013:161278. doi: 10.1155/2013/161278. Epub 2013 Apr 11.
Disturbance in the gut microbial niche by antibiotics like neomycin produces gastrointestinal (GI) disorders. Here, we evaluated the impact of a mixture of extracts of three herbs (Atractylodis Rhizoma Macrocephalae, Massa Medicata Fermentata, and Dolichoris Semen) with known GI protective activities, either laboratory unfermented (herbal formulation-1 (HF-1)) or fermented/re-fermented (herbal formulation-2 (HF-2)) on neomycin-treated rats using a commercial Lactobacillus probiotic as a reference. Treatment with neomycin augmented stool water content, decreased fecal population of Lactobacillus spp., changed the histology of intestine without inducing inflammation, reduced the colonic expression of zonula occludens-1 (ZO-1) and claudin-1, and elevated the serum C-reactive protein (CRP) and interferon-gamma (IFN- γ ) levels. Coadministration of either HF-2 or probiotic, but not HF-1, restored the fecal content of Lactobacillus spp., normalized the serum CRP level, and significantly increased the colonic expression of ZO-1 and claudin-1 in neomycin-treated rats. The combined treatment with any of the above agents ameliorated the histological changes of cecum and colon in neomycin-treated rats, and the magnitude of this effect was probiotic > HF-2 > HF-1. Our study revealed the intestinal protective effect of a mixture of three herbs against neomycin insult, which is mediated through multiple mechanisms and is potentiated upon prior fermentation/refermentation of the herbs.
抗生素（如新霉素）扰乱肠道微生物生态位会产生胃肠道（GI）疾病。在这里，我们评估了三种草药（白术、神曲和猪苓）提取物混合物的影响，这些草药具有已知的胃肠道保护作用，无论是实验室未发酵（草药配方 1 (HF-1)）还是发酵/再发酵（草药配方 2 (HF-2)），并用一种商业乳酸杆菌益生菌作为参考，用于新霉素处理的大鼠。新霉素处理增加了粪便含水量，降低了粪便中乳酸杆菌属的数量，改变了肠道组织学而没有引起炎症，降低了结肠闭合蛋白-1 (ZO-1) 和紧密连接蛋白-1 (claudin-1) 的表达，并升高了血清 C 反应蛋白 (CRP) 和干扰素-γ (IFN-γ) 水平。HF-2 或益生菌的共同给药，但不是 HF-1，恢复了新霉素处理大鼠粪便中乳酸杆菌属的含量，使血清 CRP 水平正常化，并显著增加了新霉素处理大鼠结肠中 ZO-1 和 claudin-1 的表达。上述任何一种药物的联合治疗都改善了新霉素处理大鼠盲肠和结肠的组织学变化，这种作用的程度是益生菌 > HF-2 > HF-1。我们的研究揭示了三种草药混合物对新霉素损伤的肠道保护作用，这是通过多种机制介导的，并且在草药的预先发酵/再发酵后得到增强。
