利用光子相关光谱法评估公牛精子的活力

Motility evaluation of bull spermatozoa by photon correlation spectroscopy.

作者信息

Cooke D F, Hallett F R, Barker C A

出版信息

J Mechanochem Cell Motil. 1976;3(4):219-23.

Abstract

Quasi-elastic light scattering techniques are employed to evaluate motility of bovine spermatozoa. The electric field correlation function CE(k, tau) has two components CE(k, tau) = alphafm+(1-alpha)fd, where fm is the correlation function due to motile cells, fd is due to dead cells and alpha is the fraction of motile cells. A function which is a linear combination of the experimentally measured fd and an empirically determined function fm is fit to the CE(k, tau) data. In this way, alpha and the approximate analytic form of fm are determined. The distribution of swimming speeds P(v) is derived from fm using an inverse Fourier sine transform procedure. Results for the time dependence of motility of bull sperm in Hank's balanced salt solution are presented.

摘要

采用准弹性光散射技术评估牛精子的活力。电场相关函数CE(k, τ)有两个分量CE(k, τ)=αfm+(1 - α)fd，其中fm是活细胞的相关函数，fd是死细胞的相关函数，α是活细胞的比例。将实验测量的fd与经验确定的函数fm的线性组合函数拟合到CE(k, τ)数据。通过这种方式，确定了α和fm的近似解析形式。利用傅里叶正弦逆变换程序从fm导出游动速度分布P(v)。给出了公牛精子在汉克平衡盐溶液中活力随时间变化的结果。