通过准弹性光散射测定的公牛精子的游动速度分布。
Swimming speed distributions of bull spermatozoa as determined by quasi-elastic light scattering.
作者信息
Hallett F R, Craig T, Marsh J
出版信息
Biophys J. 1978 Mar;21(3):203-16. doi: 10.1016/S0006-3495(78)85520-9.
88 semen samples from 39 bulls have been investigated by the quasi-elastic light scattering technique. Normal, defective, and dead cells each yielded characteristic autocorrelation functions. The form of these functions indicates that the swimming speed distribution of normal cells is a gamma distribution with two degrees of freedom while that for defective or circular swimmers is a gamma distribution with one degree of freedom. The resulting analysis of the experimental autocorrelation functions yields the fraction of the sample that is normal, the fraction that is defective, and the average speed of each group. The average helical swimming speed of normal cells was found to be 384 micron/s, while the average trajectory speed of the circular swimmers was found to be 103 micron/s. The overall quality of the semen samples as determined by light scattering is compared to quality determination on the same samples by technicians from the artificial insemination industry.
利用准弹性光散射技术对来自39头公牛的88份精液样本进行了研究。正常细胞、有缺陷细胞和死亡细胞各自产生了特征性的自相关函数。这些函数的形式表明，正常细胞的游动速度分布是具有两个自由度的伽马分布，而有缺陷或呈圆形游动的细胞的游动速度分布是具有一个自由度的伽马分布。对实验自相关函数的分析结果得出了样本中正常部分的比例、有缺陷部分的比例以及每组的平均速度。发现正常细胞的平均螺旋游动速度为384微米/秒，而圆形游动细胞的平均轨迹速度为103微米/秒。将通过光散射测定的精液样本的整体质量与人工授精行业技术人员对相同样本的质量测定结果进行了比较。
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