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通过光子相关光谱法评估人类精子的活力

Motility evaluation of human spermatozoa by photon correlation spectroscopy.

作者信息

Finsy R, Peetermans J, Lekkerkerker H

出版信息

Biophys J. 1979 Aug;27(2):187-91. doi: 10.1016/S0006-3495(79)85210-8.

Abstract

The application of photon correlation spectroscopy for the evaluation of motility parameters of undiluted human sperm is investigated. Measurements on semen samples, selected visually as good (i.e., fraction motile spermatozoa larger than 0.6 and a positive appreciation of the motion), gave estimates of the fraction motile spermatozoa, reproducible within 10%, and of the mean velocity of the motile cells, reproducible within 5%.

摘要

研究了光子相关光谱法在评估未稀释人类精子运动参数方面的应用。对通过肉眼挑选出的优质精液样本(即活动精子比例大于0.6且运动表现良好)进行测量,得出的活动精子比例估计值的重现性在10%以内,活动细胞平均速度的重现性在5%以内。

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本文引用的文献

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Spectral analysis of laser light scattered from motile microorganisms.
Biophys J. 1971 Apr;11(4):341-54. doi: 10.1016/S0006-3495(71)86219-7.
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Application of splines to the calculation of bacterial swimming speed distributions.
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