Craig T, Hallett F R, Nickel B
Biophys J. 1982 Apr;38(1):63-70. doi: 10.1016/S0006-3495(82)84531-1.
The Rayleigh-Gans-Debye approximation is used to predict the electric field autocorrelation functions of light scattered from circularly swimming bull spermatozoa. Using parameters determined from cinematography and modeling the cells as coated ellipsoids of semiaxes a = 0.5 micrometers, b = 2.3 micrometers, and c = 9.0 micrometers, we were able to obtain model spectra that mimic the data exactly. A coat is found to be a necessary attribute of the particle. It is also clear that these model functions at 15 degrees may be represented by the relatively simple function used before by Hallett et al. (1978) to fit data from circularly swimming cells, thus giving some physical meaning to these functional shapes. Because of this agreement the half-widths of experimental functions can now be interpreted in terms of an oscillatory frequency for the movement of the circularly swimming cell. The cinematographic results show a trend to chaotic behavior as the temperature of the sample is increased, with concomitant decrease in overall efficiency. This is manifested by a decrease in oscillatory frequency and translational speed.
瑞利 - 甘斯 - 德拜近似法用于预测从做圆周运动的公牛精子散射光的电场自相关函数。利用从电影摄影术中确定的参数，并将细胞建模为半轴分别为a = 0.5微米、b = 2.3微米和c = 9.0微米的包覆椭球体，我们能够获得与数据精确匹配的模型光谱。发现包膜是粒子的一个必要属性。同样明显的是，这些在15度时的模型函数可以由哈雷特等人（1978年）之前用于拟合做圆周运动细胞数据的相对简单的函数表示，从而赋予这些函数形状一些物理意义。由于这种一致性，现在可以根据做圆周运动细胞运动的振荡频率来解释实验函数的半高宽。电影摄影结果表明，随着样品温度升高，存在向混沌行为发展的趋势，同时整体效率降低。这表现为振荡频率和平移速度的降低。
