Harvey J D, Woolford M W
Biophys J. 1980 Jul;31(1):147-56. doi: 10.1016/S0006-3495(80)85046-6.
Calculations based on the known dimensions of bull spermatozoa show that the scattered light intensity is strongly dependent upon the relative orientation of the particle to the incident beam. The magnitude of this effect of apparently much greater than for other systems where motility has been investigated by dynamic light scattering. The calculations show that the scattering source can be approximated by a small spinning mirror, and consequently the greatest light intensity at the detector results from cells swimming in a direction perpendicular to the scattering vector. The calculations are in substantial agreement with photographic observations, as well as direct measurements of the scattered intensity. Previous treatments of dynamic light scattering from swimming bull spermatozoa based on point scattering models are shown to be incorrect.
基于已知的公牛精子尺寸进行的计算表明，散射光强度强烈依赖于粒子相对于入射光束的相对取向。这种效应的大小显然比通过动态光散射研究运动性的其他系统要大得多。计算表明，散射源可以近似为一个小的旋转镜，因此探测器处的最大光强来自于沿垂直于散射矢量方向游动的细胞。这些计算与摄影观测以及散射强度的直接测量结果基本一致。以前基于点散射模型对游动公牛精子的动态光散射处理被证明是不正确的。
