在选定细胞位点对单分子周围膜电位进行非线性光学测量。
Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites.
作者信息
Peleg G, Lewis A, Linial M, Loew L M
机构信息
Division of Applied Physics, Center for Neural Computation, Department of Ophthalmology, Hadassah Laser Center, Hebrew University of Jerusalem, Jerusalem 91904, Israel.
出版信息
Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6700-4. doi: 10.1073/pnas.96.12.6700.
Abstract
Membrane potential around single molecules has been measured by using the nonlinear optical phenomenon of second harmonic generation. This advance results from the interaction between a highly dipolar molecule with a selectively directed highly polarizable 1-nm gold particle. With this approach, a second harmonic signal, which is enhanced by the nanoparticle, is detected from a volume of nanometric dimensions. This present work clearly shows that functional cellular imaging around single molecules is possible by selectively directing an antibody with a 1-nm gold label to a specific membrane protein. The results of this work open the way for three-dimensional, high resolution functional imaging of membrane electrophysiology in cells and cellular networks.
摘要
利用二次谐波产生的非线性光学现象测量了单个分子周围的膜电位。这一进展源于一个具有高度偶极矩的分子与一个选择性定向的、高度可极化的1纳米金颗粒之间的相互作用。通过这种方法，从纳米尺寸的体积中检测到由纳米颗粒增强的二次谐波信号。这项工作清楚地表明，通过将带有1纳米金标记的抗体选择性地导向特定的膜蛋白，可以实现围绕单个分子的功能性细胞成像。这项工作的结果为细胞和细胞网络中膜电生理学的三维高分辨率功能成像开辟了道路。
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