Huang J Y, Lewis A, Loew L
Department of Applied Physics, Cornell University, Ithaca, New York 14853.
Biophys J. 1988 May;53(5):665-70. doi: 10.1016/S0006-3495(88)83147-3.
The nonlinear optical properties of dyes that alter their optical characteristics rapidly with membrane potential are described. The second harmonic signals from these dyes characterized in this paper are among the largest that have been detected to date. Structural conclusions are drawn from the second harmonic signals generated by the Langmuir Blodgett monolayers used in these measurements. Our results indicate that with appropriate instrumentation second harmonic signals could readily be detected from living cells stained with these dyes.
描述了随着膜电位快速改变其光学特性的染料的非线性光学性质。本文所表征的这些染料的二次谐波信号是迄今为止所检测到的最大信号之一。从这些测量中使用的朗缪尔-布洛杰特单分子层产生的二次谐波信号得出了结构结论。我们的结果表明,使用适当的仪器,很容易从用这些染料染色的活细胞中检测到二次谐波信号。