将各部分组合起来：绘制 O-糖蛋白组图谱。
Putting the pieces together: mapping the O-glycoproteome.
机构信息
Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
出版信息
Curr Opin Biotechnol. 2021 Oct;71:130-136. doi: 10.1016/j.copbio.2021.07.006. Epub 2021 Aug 4.
Protein glycosylation is the most diverse and omnipresent protein modification. Glycosylation provides glycoproteins with important structural and functional properties to facilitate critical biological processes. Despite the significance of protein glycosylation, the investigation of glycoproteome, especially O-linked glycoproteome, remains elusive due to the lack of a comprehensive methodology to conform with the diversity of O-linked glycoforms of O-linked glycoproteins. In recent years, mass spectrometry has become an indispensable tool for the characterization of O-linked glycosylated proteins across biological systems. We herein highlight the recent developments in MS-based O-linked glycoproteomic technologies, quantitative data acquisition strategy and bioinformatic tools, with a special focus on mucin-type O-linked glycosylation.
蛋白质糖基化是最具多样性和普遍性的蛋白质修饰。糖基化赋予糖蛋白重要的结构和功能特性，以促进关键的生物过程。尽管蛋白质糖基化意义重大，但糖蛋白质组学的研究，特别是 O-连接糖蛋白质组学的研究仍然难以捉摸，因为缺乏一种全面的方法来符合 O-连接糖蛋白 O-连接糖型的多样性。近年来，质谱已成为在生物系统中对 O-连接糖蛋白进行特征分析的不可或缺的工具。我们在此重点介绍基于 MS 的 O-连接糖蛋白质组学技术、定量数据采集策略和生物信息学工具的最新进展，特别关注粘蛋白型 O-连接糖基化。
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