Ostergaard K, Andersen C B, Petersen J, Bendtzen K, Salter D M
Osteoarthritis Research Unit, Institute for Inflammation Research, National University Hospital/Rigshospitalet, Copenhagen, Denmark.
Ann Rheum Dis. 1999 Apr;58(4):208-13. doi: 10.1136/ard.58.4.208.
To determine the validity of the histological-histochemical grading system (HHGS) for osteoarthritic (OA) articular cartilage.
Human articular cartilage was obtained from macroscopically normal (n = 13) and OA (n = 21) knee joints. Sections of central and peripheral regions of normal samples were produced. Sections of regions containing severe, moderate, and mild OA changes were produced from each OA sample. A total of 89 sections were graded by means of the HHGS (0-14) twice by three observers.
Average scores for regions designated severe (8.64) and moderate (5.83) OA were less than the expected (10-14 and 6-9, respectively) according to the HHGS, whereas average scores for the region designated mild (5.29) OA and central and peripheral regions (2.19) of normal cartilage were higher than expected (2-5 and 0-1, respectively). The HHGS was capable of differentiating between articular cartilage from macroscopically normal and OA joints and between the region designated severe OA and other regions. However, the HHGS did not adequately differentiate between regions designated mild and moderate OA. Values for sensitivity, specificity, and efficiency for all regions varied considerably.
The HHGS is valid for normal and severe OA cartilage, but does not permit distinction between mild and moderate OA changes in articular cartilage.
确定组织学 - 组织化学分级系统（HHGS）对骨关节炎（OA）关节软骨的有效性。
从宏观正常（n = 13）和骨关节炎（n = 21）膝关节获取人类关节软骨。制备正常样本中央和周边区域的切片。从每个骨关节炎样本中制备包含重度、中度和轻度骨关节炎变化区域的切片。三位观察者对总共89个切片进行两次HHGS（0 - 14）分级。
根据HHGS，指定为重度（8.64）和中度（5.83）骨关节炎区域的平均得分低于预期（分别为10 - 14和6 - 9），而指定为轻度（5.29）骨关节炎区域以及正常软骨的中央和周边区域（2.19）的平均得分高于预期（分别为2 - 5和0 - 1）。HHGS能够区分宏观正常和骨关节炎关节的关节软骨，以及区分指定为重度骨关节炎的区域和其他区域。然而，HHGS不能充分区分指定为轻度和中度骨关节炎的区域。所有区域的敏感性、特异性和效率值差异很大。
HHGS对正常和重度骨关节炎软骨有效，但不能区分关节软骨中轻度和中度骨关节炎变化。
