Bowen R A, Wierzbicki A A, Clandinin M T
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
Pediatr Res. 1999 Jun;45(6):815-9. doi: 10.1203/00006450-199906000-00006.
The objective of this study was to investigate if increasing maternal dietary linolenic acid (18:3n-3) content, by decreasing the 18:2n-6 to 18:3n-3 ratio, could increase the docosahexaenoic acid (22:6n-3) content in phospholipids of neuronal cells of rat pups at 2 weeks of age. Sprague-Dawley dams at parturition were fed semipurified diets containing decreasing ratios of 18:2n-6 to 18:3n-3 from 21.6:1 to 1:1. During the first 2 weeks of life, the rat pups received only their dam's milk. The fatty acid composition of the pups stomach contents (dam's milk) and the phospholipids from neuronal cells were identified and quantitated by gas-liquid chromatography. The stomach 22:6n-3 content analyzed from the rat pups at 2 weeks of age was altered by the maternal diet. Fatty acid analysis of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS) in neuronal cells of the rat pups showed no significant increase in 22:6n-3 content with increasing 18:3n-3 in the maternal diet (p > 0.05). In contrast, the content of 22:6n-3 in phosphatidylinositol (PI) was significantly increased by change in dietary 18:3n-3 intake from a dietary 18:2n-6 to 18:3n-3 ratio of 7.8:1 to 4.4:1. It is concluded that increasing maternal dietary 18:3n-3 by decreasing the 18:2n-6 to 18:3n-3 ratio does not significantly increase the 22:6n-3 content in PC, PE, and PS in neuronal cells of rat pups at 2 weeks of age.
本研究的目的是调查通过降低18:2n-6与18:3n-3的比例来增加母体饮食中亚麻酸（18:3n-3）的含量，是否能增加2周龄大鼠幼崽神经细胞磷脂中二十二碳六烯酸（22:6n-3）的含量。分娩时的斯普拉格-道利母鼠被喂食半纯化日粮，其中18:2n-6与18:3n-3的比例从21.6:1降至1:1。在出生后的前2周，大鼠幼崽仅食用母鼠的乳汁。通过气液色谱法对幼崽胃内容物（母鼠乳汁）和神经细胞磷脂的脂肪酸组成进行鉴定和定量。2周龄大鼠幼崽胃中22:6n-3的含量受母体饮食的影响。对大鼠幼崽神经细胞中磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和磷脂酰丝氨酸（PS）的脂肪酸分析表明，随着母体饮食中18:3n-3含量的增加，22:6n-3的含量没有显著增加（p>0.05）。相比之下，当饮食中18:3n-3的摄入量从18:2n-6与18:3n-3比例为7.8:1变为4.4:1时，磷脂酰肌醇（PI）中22:6n-3的含量显著增加。得出的结论是，通过降低18:2n-6与18:3n-3的比例来增加母体饮食中18:3n-3的含量，并不会显著增加2周龄大鼠幼崽神经细胞中PC、PE和PS中22:6n-3的含量。
