Li Y, Su J J, Qin L L, Yang C, Ban K C, Yan R Q
Department of Pathology, Guangxi Cancer Institute, P R China.
Ann Acad Med Singap. 1999 Jan;28(1):67-71.
An animal experiment with tree shrews was performed to detect the synergistic effects of hepatitis B virus (HBV) infection and dietary aflatoxin B1 (AFB1) in hepatocarcinogenesis. Adult healthy tree shrews (Tupaia belangeri chinensis) were divided into four groups: Group A (HBV + AFB1)--animals were infected with human HBV serum at first, then fed AFB1 diluted with milk, 150 ug/kg.bw/day, 6 days/week for 105 weeks. Group B (HBV)--animals were infected with human HBV as Group A, but no AFB1 treatment. Group C (AFB1)--animals were treated with AFB1 as Group A but no HBV infection. Group D--animals were treated neither with human HBV nor AFB1. During the experiment, blood samples and liver biopsies were taken regularly from all animals in each group. All the animals were sacrificed on the 160th week when the experiment ended. The samples of sera and liver tissues were checked for HBV markers and histological changes. Hepatocellular carcinomas (HCCs) were found only in Group A and Group C, with incidences of 67% and 30% respectively. The average time for HCC occurrence in Group A and Group C was 120.3 +/- 16.6 and 153.3 +/- 5.8 weeks respectively (P < 0.01). Even though no HCC occurred in Group B, 1 animal which died before the end of the experiment showed two large hepatocellular nodules. These results showed that there is synergistic effect between HBV and AFB1 in tree shrews' hepatocarcinogenesis, even though the hepatocarcinogenic effect played by HBV alone is rather weak.
进行了一项树鼩动物实验，以检测乙型肝炎病毒（HBV）感染与膳食黄曲霉毒素B1（AFB1）在肝癌发生过程中的协同作用。将成年健康树鼩（Tupaia belangeri chinensis）分为四组：A组（HBV + AFB1）——动物首先感染人HBV血清，然后喂食用牛奶稀释的AFB1，150μg/kg体重/天，每周6天，共105周。B组（HBV）——动物与A组一样感染人HBV，但未进行AFB1处理。C组（AFB1）——动物与A组一样接受AFB1处理，但未感染HBV。D组——动物既未接受人HBV处理，也未接受AFB1处理。在实验过程中，定期从每组所有动物采集血样和肝活检组织。实验结束时，在第160周处死所有动物。检查血清和肝组织样本中的HBV标志物及组织学变化。仅在A组和C组发现肝细胞癌（HCC），发生率分别为67%和30%。A组和C组HCC发生的平均时间分别为120.3±16.6周和153.3±5.8周（P<0.01）。尽管B组未发生HCC，但1只在实验结束前死亡的动物出现了两个大的肝细胞结节。这些结果表明，HBV与AFB1在树鼩肝癌发生过程中存在协同作用，尽管单独由HBV发挥的致癌作用相当微弱。
