Yan R Q, Su J J, Huang D R, Gan Y C, Yang C, Huang G H
Department of Pathology, Guangxi Institute for Research on Cancer, Nanning, P. R. China.
J Cancer Res Clin Oncol. 1996;122(5):289-95. doi: 10.1007/BF01261405.
On the basis of the successful establishment of an animal model in tree shrews experimentally infected with human hepatitis B virus (HBV), a study on the hepatocarcinogenic effects of HBV and/or aflatoxin B1 (AFB1) was conducted. The results showed that the incidence of hepatocellular carcinoma (HCC) was significantly higher in the animals both infected with HBV and exposed to AFB1 (52.94%) than in those solely infected with HBV (11.11%) or exposed to AFB1 (12.50%). No HCC of precancerous lesions were found in the controls that were neither HBV-infected nor AFB-1 exposed. Precancerous lesions, including liver cell dysplasia and enzyme-altered hyperplastic hepatocyte foci, were observed before the occurrence of HCC, and the frequency of their appearance correlated well with the incidence of HCC. HBV DNA and the protein it encodes were detected in the cancer cells and/or the surrounding hepatocytes. Integration of HBV DNA into the host liver genome was found during hepatocarcinogenesis among the animals infected by HBV. These results suggest that exposure to HBV and AFB1 may play a synergistic role in the development of HCC, and support the viewpoint of an aetiological relationship between HBV and HCC.
在成功建立树鼩感染人乙型肝炎病毒（HBV）的动物模型的基础上，开展了关于HBV和/或黄曲霉毒素B1（AFB1）致癌作用的研究。结果显示，同时感染HBV并暴露于AFB1的动物中肝细胞癌（HCC）的发生率（52.94%）显著高于单纯感染HBV的动物（11.11%）或单纯暴露于AFB1的动物（12.50%）。在既未感染HBV也未暴露于AFB1的对照组中未发现癌前病变的HCC。在HCC发生之前观察到了包括肝细胞发育异常和酶改变的增生性肝细胞灶在内的癌前病变，其出现频率与HCC的发生率密切相关。在癌细胞和/或周围肝细胞中检测到了HBV DNA及其编码的蛋白质。在HBV感染的动物的肝癌发生过程中发现了HBV DNA整合到宿主肝脏基因组中。这些结果表明，暴露于HBV和AFB1可能在HCC的发生发展中起协同作用，并支持HBV与HCC之间病因学关系的观点。
