Hassanin A, Douzery E J
Laboratoire de Préhistoire UMR 6569 CNRS, Paris, France.
Proc Biol Sci. 1999 May 7;266(1422):893-900. doi: 10.1098/rspb.1999.0720.
To elucidate the systematic status of the enigmatic saola (Pseudoryx nghetinhensis), a new bovid genus recently discovered in Vietnam, and to investigate phylogenetic relationships within the family Bovidae, four distinct DNA markers were sequenced. Complete mitochondrial cytochrome b (1143 bp) and 12S rRNA (956 bp) genes and non-coding regions from the nuclear genes for aromatase cytochrome P-450 (199 bp) and lactoferrin (338 bp) have been compared for 25 bovid species and three Cervidae and Antilocapridae outgroups. Independent and/or combined analyses of the four nucleotide matrices through maximum parsimony and maximum-likelihood methods indicated that Bovidae consists of two major lineages, i.e. Bovinac which contains the tribes Bovini, Boselaphini and Tragelaphini, and Antilopinae which encompasses all other bovids. Within Bovinae, the tribe Bovini is divided into buffalo Bovini (Bubalus and Syncerus) and cattle Bovini (Bos and Bison) and Tragelaphini are possibly related to Boselaphini. Pseudoryx is shown to be (i) robustly nested within Bovinae; (ii) strongly associated with Bovini; and (iii) tentatively sharing a sister-group relationship with cattle Bovini. Within Antilopinae, three robust clades are in evidence: (i) Hippotragus and Damaliscus are linked to Ovis; (ii) Aepyceros joins Neotragus; and (iii) Cephalophus clusters with Oreotragus.
为阐明神秘的中南大羚（Pseudoryx nghetinhensis，一种最近在越南发现的新牛科动物属）的系统地位，并研究牛科动物内部的系统发育关系，对四个不同的DNA标记进行了测序。已对25种牛科动物以及三个鹿科和叉角羚科外群的完整线粒体细胞色素b（1143 bp）和12S rRNA（956 bp）基因以及来自核基因的芳香化酶细胞色素P - 450（199 bp）和乳铁蛋白（338 bp）的非编码区进行了比较。通过最大简约法和最大似然法对四个核苷酸矩阵进行独立和/或联合分析表明，牛科由两个主要谱系组成，即包含牛族、蓝牛族和薮羚族的牛亚科，以及包含所有其他牛科动物的羚羊亚科。在牛亚科内部，牛族分为水牛牛族（水牛属和非洲野水牛属）和黄牛牛族（牛属和美洲野牛属），薮羚族可能与蓝牛族有关。中南大羚被证明：（i）稳固地嵌套在牛亚科内；（ii）与牛族密切相关；（iii）初步与黄牛牛族具有姐妹群关系。在羚羊亚科内，有三个稳固的分支：（i）马羚属和转角牛羚属与绵羊属有联系；（ii）黑斑羚属与新小羚属相连；（iii）麂羚属与岩羚属聚类。
