Kearns R J, Hinrichs D J
Infect Immun. 1978 Dec;22(3):676-80. doi: 10.1128/iai.22.3.676-680.1978.
A water-soluble extract of Listeria monocytogenes strain 10403 acts as a mitogen on cultured mouse spleen lymphocytes. This mitogen induced a response six to nine times that of controls, as measured by [3H]thymidine incorporation. The mitogen extract was derived from washed bacterial cells which were mechanically disrupted with a French press. The extract was centrifuged at 105,000 X g and filtered through a 0.22-micrometer filter. Similar levels of lymphocyte stimulation were observed in lymphocyte cultures prepared from spleens of nude mice, indicating the effect of this mitogen on B-cells. The mitogenic property of this extract was destroyed by heating to 56 degrees C. This heat treatment does not destroy the antigens in the extract, which stimulate spleen cell cultures obtained from specifically immune mice. Similarly prepared extracts from Staphylococcus epidermidis and Salmonella typhimurium did not show similar levels of mitogenic activity. The mitogenic property of the L. monocytogenes extract was present in two strains of Listeria tested and was not associated with mouse virulence.
单核细胞增生李斯特菌10403菌株的水溶性提取物可作为培养的小鼠脾淋巴细胞的促有丝分裂原。通过[3H]胸苷掺入法测定，这种促有丝分裂原诱导的反应是对照的6至9倍。促有丝分裂原提取物来自经洗涤的细菌细胞，这些细胞用法国压榨机进行机械破碎。提取物在105,000×g下离心，并用0.22微米滤器过滤。在从裸鼠脾脏制备的淋巴细胞培养物中观察到相似水平的淋巴细胞刺激，表明这种促有丝分裂原对B细胞的作用。该提取物的促有丝分裂特性在加热至56℃时被破坏。这种热处理不会破坏提取物中的抗原，这些抗原可刺激从特异性免疫小鼠获得的脾细胞培养物。从表皮葡萄球菌和鼠伤寒沙门氏菌制备的类似提取物未显示出类似水平的促有丝分裂活性。单核细胞增生李斯特菌提取物的促有丝分裂特性在所测试的两株李斯特菌中均存在，且与小鼠毒力无关。
