Pier G B, Sidberry H F, Sadoff J C
Infect Immun. 1978 Dec;22(3):919-25. doi: 10.1128/iai.22.3.919-925.1978.
A high-molecular-weight alkali-labile polysaccharide (PS) isolated from the slime of immunotype 1 Pseudomonas aeruginosa was tested for its ability to protect mice from lethal challenge with the live, homologous organism. Intraperitoneal (i.p.) injection of 10 to 25 microgram of the PS protected 60 to 70% of the mice against challenge with up to 50 50% lethal dose units. Although single immunization of mice with up to 250 microgram of PS effected protective levels of only 70%, two successive immunizations provided 100% protection. Subcutaneous and intravenous immunization with PS also provided protection to i.p. challenges with immunotype 1 P. aeruginosa, but not to i.p. challenge with immunotype 4 P. aeruginosa. Although lipopolysaccharide (LPS) was found to be more immunogenic than PS in out studies, contamination of the alkali-labile PS with LPS did not account for the protection seen. Alkali treatment (0.1 N NaOH, 37 degrees C, 2 h) of the PS destroyed its protective effectiveness, while similarly treated LPS retained its capacity for inducing immunity in mice. Adsorption and passive protection studies with sera raised to either PS or a mixture of PS and LPS indicated that antibody directed to the alkali-labile PS antigen was capable of contributing to the protection of mice against challenge with P. aeruginosa.
从1型铜绿假单胞菌黏液中分离出的一种高分子量碱不稳定多糖（PS），对其保护小鼠免受活的同源菌株致死性攻击的能力进行了测试。腹腔注射10至25微克的PS可保护60%至70%的小鼠免受高达50个50%致死剂量单位的攻击。尽管用高达250微克的PS对小鼠进行单次免疫的保护水平仅为70%，但连续两次免疫可提供100%的保护。用PS进行皮下和静脉免疫也能保护小鼠免受1型铜绿假单胞菌的腹腔攻击，但不能保护免受4型铜绿假单胞菌的腹腔攻击。尽管在我们的研究中发现脂多糖（LPS）比PS更具免疫原性，但碱不稳定PS被LPS污染并不能解释所观察到的保护作用。对PS进行碱处理（0.1N NaOH，37℃，2小时）会破坏其保护效果，而同样处理的LPS仍保留其在小鼠中诱导免疫的能力。用针对PS或PS与LPS混合物产生的血清进行吸附和被动保护研究表明，针对碱不稳定PS抗原的抗体能够有助于保护小鼠免受铜绿假单胞菌的攻击。
