ZAM是一种来自黑腹果蝇的反转录元件，其整合机制可作为一种序列特异性内切核酸酶发挥作用。
The integration machinery of ZAM, a retroelement from Drosophila melanogaster, acts as a sequence-specific endonuclease.
作者信息
Leblanc P, Dastugue B, Vaury C
机构信息
Unité INSERM U384, 63001 Clermont-Ferrand, France.
出版信息
J Virol. 1999 Aug;73(8):7061-4. doi: 10.1128/JVI.73.8.7061-7064.1999.
Abstract
Retroviruses and retrotransposons insert into the host genome with no obvious sequence specificity. We examined the target sites of the retroelement ZAM by sequencing each host-ZAM junction in the genome of Drosophila melanogaster. Our overall data provide compelling evidence that ZAM integration machinery recognizes and leads to ZAM insertion into the sequence 5'-GCGCGCg-3'. This unique property of ZAM will facilitate the development of new tools to study the integration process of retroelements.
摘要
逆转录病毒和逆转座子插入宿主基因组时没有明显的序列特异性。我们通过对黑腹果蝇基因组中每个宿主 - ZAM连接点进行测序，研究了逆转元件ZAM的靶位点。我们的总体数据提供了令人信服的证据，表明ZAM整合机制识别并导致ZAM插入到序列5'-GCGCGCg-3'中。ZAM的这一独特特性将有助于开发研究逆转元件整合过程的新工具。
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