Peracchia M T, Harnisch S, Pinto-Alphandary H, Gulik A, Dedieu J C, Desmaële D, d'Angelo J, Müller R H, Couvreur P
Université Paris-Sud XI, URA CNRS 1218-Physico-Chimie, Pharmacotechnie, Biopharmacie, Châtenay-Malabry, France.
Biomaterials. 1999 Jul;20(14):1269-75. doi: 10.1016/s0142-9612(99)00021-6.
The in vitro protein-rejecting properties of PEG-coated polyalkylcyanoacrylate (PACA) nanoparticles were for the first time visualized after freeze-fracture of the nanoparticles pre-incubated with fibrinogen as a model blood protein. The reduced protein association to the nanoparticles was evidenced also by two-dimensional PAGE after incubation of the nanoparticles with human plasma. In vivo experiments showed the 'stealth' long-circulating properties of the PEGylated nanoparticles after intravenous administration to mice. Thus, the images obtained after nanoparticle-protein incubation were predictive of the behavior observed in vivo. In conclusion, freeze-fracture analysis represents a novel and original qualitative approach to investigate the interactions between proteins and particulate systems.
