Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA, 02114, USA.
Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu City, 300, Taiwan.
Adv Healthc Mater. 2022 Jan;11(2):e2102035. doi: 10.1002/adhm.202102035. Epub 2021 Nov 16.
Magnetic nanoparticles with hybrid sensing functions are in wide use for bioseparation, sensing, and in vivo imaging. Yet, nonspecific protein adsorption to the particle surface continues to present a technical challenge and diminishes the theoretical protein detection capabilities. Here, a magneto-plasmonic nanoparticle synthesis is developed that minimizes nonspecific protein adsorption. Building on the success of zwitterionic polymers, a highly stable and anergic nanomaterial, magnetic gold nanoparticles with idealized coating (MAGIC) is obtained with significantly lower serum protein adsorption compared to control nanoparticles coated with commonly used polymers (polyethylene glycol, polyethylenimine, or polyallylamine hydrochloride). MAGIC nanoparticles are able to sense specific bladder cancer biomarkers at low levels and in the presence of other proteins. This strategy may find wide spread applications for in vitro and in vivo sensing as well as isolations.
具有混合传感功能的磁性纳米粒子在生物分离、传感和体内成像方面得到了广泛应用。然而，纳米粒子表面的非特异性蛋白质吸附仍然是一个技术挑战，降低了理论上的蛋白质检测能力。在这里，开发了一种磁性等离子体纳米粒子合成方法，可最大限度地减少非特异性蛋白质吸附。基于两性离子聚合物的成功经验，得到了一种非常稳定且无反应性的纳米材料，与常用聚合物（聚乙二醇、聚乙烯亚胺或盐酸聚烯丙基胺）包覆的对照纳米粒子相比，具有理想包覆的磁性金纳米粒子（MAGIC）具有显著较低的血清蛋白吸附。MAGIC 纳米粒子能够在低水平和存在其他蛋白质的情况下检测到特定的膀胱癌生物标志物。这种策略可能在体外和体内传感以及分离方面得到广泛应用。
