Willey B M, Jones R N, McGeer A, Witte W, French G, Roberts R B, Jenkins S G, Nadler H, Low D E
Department of Microbiology, Mount Sinai Hospital, University of Toronto, Ontario, Canada.
Diagn Microbiol Infect Dis. 1999 Jul;34(3):165-71. doi: 10.1016/s0732-8893(99)00032-2.
Enterococci have become important nosocomial pathogens, with Enterococcus faecalis and then Enterococcus faecium predominating. Because of the emergence of glycopeptide (vancomycin and teicoplanin) resistance in enterococci, laboratories have been required to screen for resistant strains and to identify them to the species level. This has resulted in the need for accurate identification of species less commonly associated with clinical infections, such as Enterococcus casseliflavus and Enterococcus gallinarum, which are inherently resistant to the glycopeptides. Studies evaluating commonly used commercial identification systems, have found error rates for enterococcal species identification of 2-21% for E. faecalis, 5-9% for E. faecium, and 14-79% for other species. Reporting errors may have adverse effects on the management of clinical infections, as well as in the control of multidrug-resistant strain outbreaks. The purpose of this document is to present a simplified approach to the identification of Enterococcus species that uses a combination of rapid, readily available, and inexpensive tests.
肠球菌已成为重要的医院病原体，其中粪肠球菌和屎肠球菌占主导地位。由于肠球菌中出现了糖肽类（万古霉素和替考拉宁）耐药性，实验室需要筛查耐药菌株并将其鉴定到种水平。这就导致需要准确鉴定较少与临床感染相关的菌种，如格氏肠球菌和鹑鸡肠球菌，它们对糖肽类天然耐药。评估常用商业鉴定系统的研究发现，粪肠球菌的肠球菌种鉴定错误率为2% - 21%，屎肠球菌为5% - 9%，其他菌种为14% - 79%。报告错误可能会对临床感染的管理以及多重耐药菌株暴发的控制产生不利影响。本文档的目的是介绍一种简化的肠球菌鉴定方法，该方法使用快速、易于获得且廉价的测试组合。
