Mathis A, Tanner I, Weber R, Deplazes P
Institute of Parasitology, University of Zürich, Switzerland.
Int J Parasitol. 1999 May;29(5):767-70. doi: 10.1016/s0020-7519(99)00025-9.
Encephalitozoon hellem is a microsporidian species that causes disseminated infections in HIV-positive patients. Identical genotypes of E. hellem, as assessed by the sequence of the rDNA internal transcribed spacer, have been identified in isolates from humans and from a psittacine bird. However, by analysing the rDNA ITS of four E. hellem isolates from Switzerland (three) and Tanzania (one), two new genotypes were identified. Differences among the E. hellem isolates were also detected by Western blot analysis, but there was no absolute match between ITS genotype and antigen profile. Hence, strain variation exists in E. hellem and the ITS sequence seems a valuable marker in obtaining further insight into the epidemiology of this pathogen.
海伦脑炎微孢子虫是一种可在HIV阳性患者中引起播散性感染的微孢子虫物种。通过rDNA内转录间隔区序列评估，已在来自人类和一只鹦鹉的分离株中鉴定出相同基因型的海伦脑炎微孢子虫。然而，通过分析来自瑞士（3株）和坦桑尼亚（1株）的4株海伦脑炎微孢子虫分离株的rDNA ITS，鉴定出了两种新基因型。通过蛋白质印迹分析也检测到了海伦脑炎微孢子虫分离株之间的差异，但ITS基因型与抗原谱之间不存在绝对匹配。因此，海伦脑炎微孢子虫存在菌株变异，ITS序列似乎是进一步深入了解该病原体流行病学的一个有价值的标志物。
