Ivanovska N, Hristova M, Philipov S
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
Int J Immunopharmacol. 1999 May;21(5):337-47. doi: 10.1016/s0192-0561(99)00015-6.
The main alkaloids isopyruthaline (It1), fangchinoline (It2) and isothalictrine (It3), isolated from Isopyrum thalictroides (L.) were investigated in complement-mediated reactions. The alkaloids influenced the alternative pathway (AP) activity in normal human serum (NHS). They enhanced the inhibitory action of complement activators--carrageenan (Car), zymosan (Zy), hydrogen peroxide (HP) and high temperature via classical pathway (CP) in NHS. Substances strongly potentiated the action of zymosan and cobra venom (CV) in guinea pig serum (GPS). It was established that they could provoke C3 conversion in NHS and mouse sera (MS). The antiinflammatory properties of the alkaloids were evaluated in mouse paw oedema induced by CV, Zy and histamine (His). Isopyruthaline and isothalictrine suppressed paw swelling in CV- and Zy-oedema. They were applied in Zy-induced multiple organ dysfunction syndrome (MODS) in mice. The alkaloids inhibited the increase of the serum complement activity provoked by the injection of zymosan. Itl lowered the mortality rate of mice with MODS if its application proceeded Zy. An increase of the number of mice without tissue injury was established after treatment with It1 and It3.
对从唐松草（Isopyrum thalictroides (L.)）中分离出的主要生物碱异唐松草碱（It1）、粉防己碱（It2）和异塔利特灵（It3）在补体介导的反应中进行了研究。这些生物碱影响正常人血清（NHS）中的替代途径（AP）活性。它们增强了补体激活剂——角叉菜胶（Car）、酵母聚糖（Zy）、过氧化氢（HP）和高温通过经典途径（CP）在NHS中的抑制作用。这些物质强烈增强了酵母聚糖和眼镜蛇毒（CV）在豚鼠血清（GPS）中的作用。已证实它们可在NHS和小鼠血清（MS）中引发C3转化。在由CV、Zy和组胺（His）诱导的小鼠爪肿胀中评估了这些生物碱的抗炎特性。异唐松草碱和异塔利特灵抑制了CV和Zy诱导的爪肿胀。它们被应用于小鼠Zy诱导的多器官功能障碍综合征（MODS）。这些生物碱抑制了注射酵母聚糖引起的血清补体活性增加。如果在注射Zy后应用It1，它可降低MODS小鼠的死亡率。在用It1和It3治疗后，未出现组织损伤的小鼠数量增加。
